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DNMT3A Antibody (MA5-52484) in WB
Western Blot analysis of DNMT3A. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DNMT3A recombinant monoclonal antibody (Product # MA5-52484) with a dilution of 1:5,000, followed by HRP-conjugated goat anti-rabbit secondary antibody with a dilution of 1:20,000. Image was developed using FeQTM ECL Substrate Kit.
产品信息
MA5-52484
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293F
分类
Recombinant Monoclonal
类型
Antibody
克隆号
23GB2230
抗原
A synthesized peptide derived from human Dnmt3a (609-624AA).
偶联物
形式
Liquid
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice (domestic); Dry ice (international)
靶标信息
Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Recently, it has been shown that naturally occurring mutations of Dnmt3b gene occurs in patients with a rare autosomal recessive disorder, termed ICF (immunodeficiency, centromeric instability, and facial anomalies) syndrome.
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生物信息学
蛋白别名: AU015806; Cysteine methyltransferase DNMT3A; DNA (cytosine-5)-methyltransferase 3A; DNA (cytosine-5-)-methyltransferase 3 alpha; DNA cytosine methyltransferase 3A2; DNA methyltransferase 3A; DNA methyltransferase HsaIIIA; DNA methyltransferase MmuIIIA; DNA MTase HsaIIIA; DNA MTase MmuIIIA; Dnmt3a; W91664
基因别名: DNMT3A; DNMT3A2; M.HsaIIIA; MmuIIIA; TBRS
UniProt ID: (Human) Q9Y6K1, (Mouse) O88508, (Rat) Q1LZ53
Entrez Gene ID: (Human) 1788, (Mouse) 13435, (Rat) 444984
DNA binding
chromatin binding
DNA (cytosine-5-)-methyltransferase activity
protein binding
DNA-methyltransferase activity
identical protein binding
unmethylated CpG binding
metal ion binding
DNA (cytosine-5-)-methyltransferase activity, acting on CpG substrates
methyltransferase activity
transferase activity
DNA methyltransferase
negative regulation of transcription from RNA polymerase II promoter
mitotic cell cycle
DNA methylation
methylation-dependent chromatin silencing
regulation of gene expression by genetic imprinting
spermatogenesis
aging
response to toxic substance
response to ionizing radiation
response to lead ion
DNA methylation on cytosine within a CG sequence
positive regulation of cell death
neuron differentiation
response to estradiol
response to vitamin A
response to antidepressant
response to cocaine
DNA methylation involved in embryo development
DNA methylation involved in gamete generation
hypermethylation of CpG island
negative regulation of gene expression, epigenetic
S-adenosylhomocysteine metabolic process
S-adenosylmethioninamine metabolic process
cellular response to amino acid stimulus
cellular response to ethanol
cellular response to hypoxia
C-5 methylation of cytosine
hepatocyte apoptotic process
transcription, DNA-templated
regulation of transcription, DNA-templated
maintenance of DNA methylation
chromatin modification
methylation
DNA methylation on cytosine
response to nutrient levels
response to drug
response to ethanol
