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DTX4 Antibody (PA5-115146) in ICC/IF
Immunocytochemistry analysis of DTX4 in HepG2 cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115146) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
产品信息
PA5-115146
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human DTX4(Accession Q9Y2E6), corresponding to amino acid residues L398-C448.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2899782
产品详细信息
Antibody detects endogenous levels of total DTX4.
靶标信息
DTX4 is a regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations. It functions as an ubiquitin ligase protein in vivo, mediating 'Lys48'-linked polyubiquitination and promoting degradation of TBK1, targeting to TBK1 requires interaction with NLRP4.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: deltex 4 homolog; deltex 4, E3 ubiquitin ligase; deltex homolog 4; Deltex4; E3 ubiquitin-protein ligase DTX4; Protein deltex-4; RING finger protein 155; RING-type E3 ubiquitin transferase DTX4
基因别名: AI449438; BC044798; D930042H13; DTX4; KIAA0937; RNF155
UniProt ID: (Human) Q9Y2E6, (Mouse) Q6PDK8
Entrez Gene ID: (Human) 23220, (Mouse) 207521
ubiquitin-protein transferase activity
zinc ion binding
ligase activity
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ribosomal S6-glutamic acid ligase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
metal ion binding
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
ubiquitin-protein ligase
