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GenScript
DYKDDDDK Tag Recombinant Rabbit Monoclonal Antibody (114F12C8), HRP, MonoRab™
Application
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DYKDDDDK Tag Antibody (A01869-40) in WB
Sensitivity and specificity of MonoRab™ DYKDDDDK Tag Antibody [HRP], mAb, Rabbit (GenScript, A01869-40) to N-DYKDDDDK-tag Fusion Protein by Western blot. Loading: Lane 1 : N-terminal DYKDDDDK-tagged fusion protein (25 ng) Lane 2 : N-terminal DYKDDDDK-tagged fusion protein (10 ng) Lane 3 : N-terminal DYKDDDDK-tagged fusion protein (5 ng) Lane 4 : N-terminal DYKDDDDK-tagged fusion protein (1 ng) Primary Antibody: MonoRab™ DYKDDDDK Tag Antibody [HRP], mAb, Rabbit (GenScript, A01869-40) 1 µg/mL.
产品信息
A01869-40
产品规格
种属反应
Tag
宿主/亚型
Rabbit
/ IgG
Expression System
Mammalian cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
114F12C8
抗原
DYKDDDDK synthetic peptide coupled to KLH
偶联物
HRP
形式
Lyophilized
纯化类型
Protein A
保存液
PBS, pH 7.4, with 1% BSA
内含物
0.01% thimerosal
保存条件
-20°C or -80°C if preferred
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.
The rabbit immune system generates antibody diversity and optimizes affinity. GenScript utilizes MonoRab™ technology to generate the high affinity and specificity monoclonal rabbit antibodies. MonoRab™ DYKDDDDK Tag Antibody [HRP], mAb, Rabbit specific to DYKDDDDK tags placed at C-terminal, N-terminal and internal regions of fusion proteins. The antibody can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify DYKDDDDK fusion proteins in bacteria and mammalian cells.
靶标信息
DYKDDDDK tag, also known as FLAG tag, is a short peptide sequence that is commonly used as a protein tag to facilitate protein purification, detection, and localization. The tag consists of eight amino acids: Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys. It is often fused to the N- or C-terminus of a protein of interest, allowing for easy identification and isolation of the protein using anti-FLAG antibodies. The DYKDDDDK tag is widely used in molecular biology and biochemistry research, and has been employed in various applications, such as protein-protein interaction studies, protein expression analysis, and protein localization studies. The FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended. FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: DDDDK; DDDDK epitope tag; ECS epitope tag; ECS tag; FLAG; FLAG Tag
