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Invitrogen
DYKDDDDK Tag Monoclonal Antibody (FG4R), DyLight™ 650
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DYKDDDDK Tag Antibody (MA1-91878-D650) in ICC/IF
Immunofluorescent analysis of DYKDDDDK Tag was performed using 70% confluent HA-H3-DYKDDDDK transfected HEK-293 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with DYKDDDDK Tag Monoclonal Antibody (FG4R), DyLight 650 (Product # MA1-91878-D650) at 5 µg/mL ... View More
产品信息
MA1-91878-D650
应用
建议稀释比
已发表文章
产品规格
种属反应
Tag
已发表种属
Tag
宿主/亚型
Mouse
/ IgG2b
分类
Monoclonal
类型
Antibody
克隆号
FG4R
抗原
Synthetic peptide (DYKDDDDK) coupled to KLH.
偶联物
激发/发射光谱
651/673 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS with proprietary stabilizer
内含物
0.02% sodium azide
保存条件
4° C, do not freeze
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2537623
产品详细信息
MA1-91878-D650 has been successfully used for immunofluorescence. DyLight 650 has an excitation/emission of 652/672 nm.
Similar epitope as the Flag tag from Sigma.
靶标信息
DYKDDDDK tag, also known as FLAG tag, is a short peptide sequence that is commonly used as a protein tag to facilitate protein purification, detection, and localization. The tag consists of eight amino acids: Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys. It is often fused to the N- or C-terminus of a protein of interest, allowing for easy identification and isolation of the protein using anti-FLAG antibodies. The DYKDDDDK tag is widely used in molecular biology and biochemistry research, and has been employed in various applications, such as protein-protein interaction studies, protein expression analysis, and protein localization studies. The FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended. FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: DDDDK; DDDDK epitope tag; ECS epitope tag; ECS tag; FLAG; FLAG Tag
