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Invitrogen
Desmin Polyclonal Antibody
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Desmin Antibody (PA5-16705) in ICC/IF
Immunofluorescence analysis Desmin was performed using 70% confluent log phase RD cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Desmin Polyclonal Antibody (Product PA5-16705) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor 488 (Product # A28175) at a dilution of... View More
产品信息
PA5-16705
应用
建议稀释比
已发表文章
产品规格
已发表种属
C. elegans,
Human,
Mouse,
Rat,
Sheep
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthetic peptide mapping near the C-terminus of human desmin
偶联物
形式
Liquid
浓度
0.25 mg/mL
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
PA5-16705 targets Desmin in IHC (P), IP, and WB applications and shows reactivity with Human, mouse, and Rat samples.
The PA5-16705 immunogen is a synthetic peptide mapping near the C-terminus of human desmin.
PA5-16705 has been successfully used in immunofluorescence and western blotting analysis of Desmin in primary human hepatic stellate cells.
靶标信息
Desmin (approximately 53 kDa) exhibits a high degree of tissue specificity, its expression being predominantly confined to all types of muscle cells (cardiac, skeletal and smooth muscle). Regulation of desmin expression is stage and tissue-specific, since it is induced during terminal development of, for example, skeletal muscle cell differentiation. In skeletal en cardiac muscle cells desmin is localized in the Z-disk region and at the intercalated disk. The expression pattern of desmin in smooth muscle is much more heterogenous. Coexpression of vimentin and desmin has been observed in tumors derived from muscle tissue, i.e. rhabdomyosarcomas and leiomyosarcomas. Furthermore, during myocard dysfunction dramatic changes in the distribution of desmin have been observed.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
