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Dityrosine Antibody (MA5-45827) in WB
Western Blot analysis of Dityrosine-BSA Conjugate showing detection of 67 kDa Dityrosine protein. Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA. Lane 3: 3,5-Dibromotyrosine-BSA. Lane 4: Dityrosine-BSA. Lane 5: Bromotyrosine-BSA. Lane 6: 7-ketocholesterol-BSA. Load: 1 µg. Blocking: 5% Skim Milk in TBST. Samples were incubated with Dityrosine monoclonal antibody (Product # MA5-45827) at 1:1,000 for 2 hours at RT, followed by Goat Anti-Mouse IgG: HRP at 1:2,000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/O... View More
产品信息
MA5-45827
产品规格
种属反应
Chemical
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
7D4
抗原
Synthetic Dityrosine conjugated to Keyhole Limpet Hemocyanin (KLH).
偶联物
激发/发射光谱
482/675 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein G
保存液
95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA
内含物
no preservative
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2932281
产品详细信息
A 1:1,000 dilution of MA5-45827 was sufficient for detection of dityrosine in 1 µg of Dityrosine conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.|Specific for dityrosine modified proteins. Does not cross-react with 3,5-dibromotyrosine or bromotyrosine modified proteins.
靶标信息
Dityrosine may be a natural component of protein structure, a product of environmental stress, or a product of in vitro protein modification.Dityrosine is involved in diverse reactions that produce tyrosyl radicals, which in turn may couple to yield dityrosine.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Dityrosine; Dityrosine (DT); DT; DY
