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Invitrogen
Donkey anti-Rabbit IgG (H+L) Secondary Antibody, Qdot™ 625
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Rabbit IgG (H+L) Secondary Antibody (Q22086) in WB
Multiplexed fluorescent western blot was performed using Donkey anti-Rabbit IgG (H+L) Secondary Antibody, Qdot™ 625 (Product # Q22086). Whole cell extracts of THP1 (Lane 1, 2, 3), MCF7 (Lane 4, 5, 6), HeLa (Lane 7), HEK-293 (Lane 8), were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP03221BOX). Resolved proteins were transferred onto a nitrocellulose membrane (Product # IB23001) by i... View More
产品信息
Q22086
应用
建议稀释比
免疫印迹 (WB)
1:50-1:500
免疫组化 (IHC)
1:50
免疫细胞化学 (ICC/IF)
1:50
流式细胞分析 (Flow)
1:50
产品规格
种属反应
Rabbit
宿主/亚型
Donkey
/ IgG
分类
Polyclonal
类型
Secondary Antibody
抗原
Gamma Immunoglobins Heavy and Light chains
偶联物
激发/发射光谱
300/621 nm
查看光谱
形式
Liquid
浓度
1 µM
纯化类型
purified
保存液
0.05M borate, pH 8.3, with 1M betaine
内含物
0.05% sodium azide
保存条件
4° C, store in dark
运输条件
Ambient
RRID
AB_2556493
靶标
IgG
抗体形式
Whole Antibody
产品详细信息
Qdot nanocrystals are composed of semi-conductor material to generate a fluorescent particle which is exceptionally bright and does not photobleach. Qdot nanocrystals paired with the correct optical filters are as much as 50 times brighter than traditional organic dyes.
靶标信息
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
