Invitrogen

Dynamin 1 Monoclonal Antibody (A1A12)

Dynamin 1 Antibody in Immunocytochemistry (ICC/IF)

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Dynamin 1 Antibody (MA5-34957) in ICC/IF

Immunofluorescent analysis of Dynamin 1 in MG-63 cells (green). Samples were formalin fixed, permeabilized with 0.1% Triton X-100 in TBS (1 hour, room temperature) and blocked with 1% BSA (15 min, room temperature), incubated with Dynamin 1 monoclonal antibody (Product # MA5-34957) at a dilution of 1:50 (1 hour, room temperature), and followed by Alexa Fluor 488 Goat anti-Rabbit IgG and DAPI (blue) with a d... View More

Dynamin 1 Antibody in Flow Cytometry (Flow)

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产品信息

MA5-34957

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

免疫细胞化学 (ICC/IF)

1:50

流式细胞分析 (Flow)

1:50

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

A1A12

抗原

Recombinant protein within Human Dynamin 1 aa 555-770

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

2 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4, with 50% glycerol, 0.2% BSA

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles, store in dark

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2848863

产品详细信息

Positive Control: PC-3M cell lysates, MG-63, SiHa, A549.

靶标信息

Dynamin is a GTPase required for endocytosis of clathrin coated vesicles from the membrane. Dynamin's involvement in endocytosis was first discovered during the characterization of the temperature sensitive parylytic shibire mutation from Drosophila melanogaster. Three closely related isoforms have been identified in mammals. Dynamin1 is expressed exclusively in neurons, dynamin2 is ubiquitously expressed and dynamin3 is expressed primarily in the testes. Each of these isoforms of dynamin also has several splice variants. This suggests that each isoform/splice variant may have separate intracellular targets or function in the budding of distinct vesicle types. Common domain features of the dynamin proteins include an amino acid terminal GTPase domain, a central pleckstrin homology domain and a highly basic proline rich carboxyl terminus with several Src homology 3 (SH3) domains. These SH3 domains target the dynamin to the clathrin coated pit. Dynamin is known to interact with at least two proteins, AP2 adaptor protein complex and amphiphysin.

仅用于科研。不用于诊断过程。未经明确授权不得转售。