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Invitrogen
E-selectin Monoclonal Antibody (1.2B6)
高级验证
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
图: 1 / 6
E-selectin Antibody (MA1-22165) in ICC/IF
Immunofluorescence analysis of E-selectin was performed using 70% confluent log phase HUVEC cells treated with 100 U/mL TNF-alpha for 3 Hrs. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with E-selectin Monoclonal Antibody (1.2B6) (Product # MA1-22165) at 1... View More
产品信息
MA1-22165
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Pig
已发表种属
Human
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
1.2B6
抗原
Human E-Selectin (ELAM-1)
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_558251
产品详细信息
Not suitable for immunohistochemistry (paraffin).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
Leukocytes adhere to the blood vessel endothelium during extravasation in postcapillary venules of lymph nodes. In addition, leukocyte adhesion occurs in the capillaries and small venules at any site in the body after onset of inflammation. This response is immediate and involves a cascade of adhesion receptors. At the endothelial surface members of the selectin and immunoglobulin superfamilies participate in this cascade. Selectins are C-type cell surface lectins that play a role in leukocyte adhesion to the blood vessel wall endothelium. E-selectin (CD62E) is an endothelial cell specific selectin that is expressed only after activation with proinflammatory cytokines. In vitro experiments have shown that IL-1, TNFalpha and bacterial wall components like lipopolysaccharides induce the transcription of E-selectin in a NFkB dependent signaling cascade. E-selectin has been associated with blood vessel endothelium in diverse inflammatory situations.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: CD62 antigen-like family member E; CD62E; e selectin; E-selectin; ELAM-1; endothelial adhesion molecule 1; Endothelial leukocyte adhesion molecule 1; LECAM2; leukocyte endothelial cell adhesion molecule 2; Leukocyte-endothelial cell adhesion molecule 2; RP1-117P20.2; selectin E (endothelial adhesion molecule 1)
基因别名: CD62E; ELAM; ELAM1; ESEL; LECAM2; SELE
UniProt ID: (Human) P16581, (Pig) P98110
Entrez Gene ID: (Human) 6401, (Pig) 397508
positive regulation of receptor internalization
leukocyte migration involved in inflammatory response
inflammatory response
heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules
leukocyte cell-cell adhesion
activation of phospholipase C activity
calcium-mediated signaling
actin filament-based process
response to lipopolysaccharide
response to tumor necrosis factor
regulation of inflammatory response
leukocyte migration
leukocyte tethering or rolling
response to interleukin-1
cell adhesion
positive regulation of leukocyte tethering or rolling
