OriGene

ECT2 Monoclonal Antibody (OTI2D9), TrueMAB™

查看其他12个ECT2抗体

抗体检测数据 (3)

ECT2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ECT2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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ECT2 Antibody (TA807408) in IHC (P)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ECT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA807408)

ECT2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ECT2 Antibody in Western Blot (WB)

ECT2 Antibody in Western Blot (WB)

Product Image Gallery

ECT2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ECT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA807408)

ECT2 Antibody in Western Blot (WB) — HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ECT2 (RC211319, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-ECT2. Positive lysates LY413314 (100 µg) and LC413314 (20 µg) can be purchased separately from OriGene.

ECT2 Antibody in Western Blot (WB) — Western blot analysis of extracts (10 µg) from 1 cell line by using anti-ECT2 monoclonal antibody. (1:200)

产品信息

TA807408

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:2,000

-

免疫组化（石蜡） (IHC (P))

1:150

-

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

OTI2D9

抗原

Human recombinant protein fragment corresponding to amino acids 175-438 of human ECT2 produced in E.coli.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS with 1% BSA, 50% glycerol

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

靶标信息

The protein encoded by this gene is a transforming protein that is related to Rho-specific exchange factors and yeast cell cycle regulators. The expression of this gene is elevated with the onset of DNA synthesis and remains elevated during G2 and M phases. In situ hybridization analysis showed that expression is at a high level in cells undergoing mitosis in regenerating liver. Thus, this protein is expressed in a cell cycle-dependent manner during liver regeneration, and is thought to have an important role in the regulation of cytokinesis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: epithelial cell transforming sequence 2 oncogene; Epithelial cell-transforming sequence 2 oncogene; FLJ10461; MGC138291; Protein ECT2

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基因别名: ARHGEF31; ECT2

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UniProt ID: (Human) Q9H8V3

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Entrez Gene ID: (Human) 1894

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signal transducer activity guanyl-nucleotide exchange factor activity Rho guanyl-nucleotide exchange factor activity GTPase activator activity protein binding Rho GTPase binding protein homodimerization activity G-protein modulator protein-binding activity modulator guanyl-nucleotide exchange factor

参与通路

cell morphogenesis cytokinesis protein transport activation of protein kinase activity positive regulation of cytokinesis regulation of Rho protein signal transduction intracellular signal transduction positive regulation of protein import into nucleus positive regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of GTPase activity positive regulation of neuron differentiation regulation of protein kinase activity regulation of small GTPase mediated signal transduction protein homooligomerization regulation of attachment of spindle microtubules to kinetochore cellular response to hydrogen peroxide bicellular tight junction assembly cellular response to calcium ion cellular response to ionizing radiation activation of GTPase activity