Bioss

EGF Polyclonal Antibody

EGF Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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EGF Antibody (BS-2010R) in IHC (P)

Formalin-fixed and paraffin embedded rat kidney labeled with Anti-EGF Polyclonal Antibody, Unconjugated (bs-2010R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

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产品信息

BS-2010R

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:300-1:1,000

免疫组化（石蜡） (IHC (P))

1:200

免疫组化（冰冻） (IHC (F))

1:100-1:500

免疫细胞化学 (ICC/IF)

1:50-1:200

酶联免疫吸附实验 (ELISA)

1:500-1:1,000

产品规格

种属反应

Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

KLH conjugated synthetic peptide derived from rat EGF, amino acids 31-53.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA

内含物

0.02% ProClin 300

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

靶标信息

EGF (Epidermal growth factor) exerts its actions by binding to the EGF Receptor, a 170 kDa protein kinase. Activation of EGFR initiates diverse cellular pathways in response to toxic environmental stimuli, or to EGF binding to the receptor, the EGFR forms homo- or heterodimers with other family members. Each dimeric receptor complex initiates a distinct signaling pathway by recruiting different Src homology 2 (SH2) containing effector proteins. EGF is far and wide expressed in kidney, cerebrum, prostrate and salivary glands. EGF acts as a potent mitogenic factor and the phosphorylated receptor recruits adapter proteins like GRB2 that activates complex downstream signaling cascades. EGF activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STAT modules. Research studies suggest the protein may also play important role in activating the NF-kappa-B signaling cascade. Defects in the EGGF gene are the cause of hypomagnesemia type 4 and dysregulation has been associated with the growth and progression of certain cancers.

仅用于科研。不用于诊断过程。未经明确授权不得转售。