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Invitrogen
EGR1 Polyclonal Antibody
高级验证
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
图: 1 / 8
EGR1 Antibody (PA5-77959) in ICC/IF
Immunofluorescence analysis of EGR1 was performed using 70% confluent log phase DU 145 and A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with EGR1 Polyclonal Antibody (Product # PA5-77959) at 1:200 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A327... View More
产品信息
PA5-77959
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant protein encompassing a sequence within the N-terminus region of human EGR1. The exact sequence is proprietary.
偶联物
形式
Liquid
浓度
0.62 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7, with 20% glycerol
内含物
0.025% ProClin 300
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2735757
产品详细信息
Positive Control: EGR1-transfected 293T cells
Predicted Reactivity: Mouse (83%), Rat (85%), Sheep (91%), Rhesus Monkey (98%), Bovine (91%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
The protein encoded by this gene belongs to the EGR family of C2H2-type zinc-finger proteins. It is a nuclear protein and functions as a transcriptional regulator. The products of target genes it activates are required for differentitation and mitogenesis. Studies suggest this is a cancer suppresor gene.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: AT225; Early growth response protein 1; EGR-1; Krox-24 nuclear protein; Nerve growth factor-induced protein A; NGFI-A; Transcription factor ETR103; Transcription factor Zif268; Zinc finger protein 225; Zinc finger protein Krox-24
基因别名: A530045N19Rik; AT225; egr; Egr-1; EGR1; ETR103; G0S30; Krox-1; KROX-24; KROX24; NGF1-A; NGFI-A; NGFIA; TIS8; Zenk; Zfp-6; ZIF-268; Zif268; ZNF225
UniProt ID: (Human) P18146, (Mouse) P08046
Entrez Gene ID: (Human) 1958, (Mouse) 13653
transcription regulatory region sequence-specific DNA binding
RNA polymerase II regulatory region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
histone acetyltransferase binding
sequence-specific DNA binding
metal ion binding
RNA polymerase II core promoter sequence-specific DNA binding
transcription factor activity, RNA polymerase II core promoter proximal region sequence-specific binding
nucleic acid binding
double-stranded DNA binding
transcription factor binding
transcription regulatory region DNA binding
C2H2 zinc finger transcription factor
negative regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
response to glucose
T cell differentiation
BMP signaling pathway
response to insulin
regulation of protein sumoylation
skeletal muscle cell differentiation
regulation of apoptotic process
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
type I interferon signaling pathway
regulation of transcription from RNA polymerase II promoter in response to hypoxia
interleukin-1-mediated signaling pathway
cellular response to gamma radiation
cellular response to heparin
cellular response to mycophenolic acid
glomerular mesangial cell proliferation
positive regulation of glomerular metanephric mesangial cell proliferation
negative regulation of canonical Wnt signaling pathway
transcription, DNA-templated
regulation of transcription, DNA-templated
learning or memory
positive regulation of gene expression
positive regulation of cell death
negative regulation of apoptotic process
positive regulation of neuron apoptotic process
regulation of long-term neuronal synaptic plasticity
cellular response to organic substance
cellular response to cAMP
cellular response to gonadotropin stimulus
