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Invitrogen
EHMT2 Polyclonal Antibody
高级验证
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
图: 1 / 7
EHMT2 Antibody (PA5-78347) in ICC/IF
EHMT2 Polyclonal Antibody detects G9a protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: G9a protein stained by EHMT2 Polyclonal Antibody (Product # PA5-78347) diluted at 1:1,000. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
产品信息
PA5-78347
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant protein encompassing a sequence within the center region of human G9a. The exact sequence is proprietary.
偶联物
形式
Liquid
浓度
1.34 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7, with 20% glycerol
内含物
0.025% ProClin 300
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2735793
产品详细信息
Positive Control: 293T, A431, HeLa, HepG2, Neuro 2A, C8D30, NIH-3T3, Raw264.7, C2C12
Predicted Reactivity: Rat (99%), Bovine (100%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
A cluster of genes, BAT1-BAT5, has been localized in the vicinity of the genes for TNF alpha and TNF beta. This gene is found near this cluster; it was mapped near the gene for C2 within a 120-kb region that included a HSP70 gene pair. These genes are all within the human major histocompatibility complex class III region. This gene was thought to be two different genes, NG36 and G9a, adjacent to each other but a recent publication shows that there is only a single gene. The protein encoded by this gene is thought to be involved in intracellular protein-protein interaction. There are three alternatively spliced transcript variants of this gene but only two are fully described.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: ankyrin repeat-containing protein; DKFZp686H08213; Euchromatic histone-lysine N-methyltransferase 2; FLJ35547; G9A histone methyltransferase; H3 ly; H3-K9-Histone-Methyl-Transferase; H3-K9-HMTase 3; Histone H3-K9 methyltransferase 3; Histone-lysine N-methyltransferase EHMT2; histone-lysine N-methyltransferase, H3 lysine-9 specific 3; HLA-B associated transcript 8; HLA-B-associated transcript 8; Lysine N-methyltransferase 1C; NG36/G9a; Protein G9a
基因别名: BAT8; C6orf30; D17Ertd710e; EHMT2; G9A; GAT8; KMT1C; NG36
UniProt ID: (Human) Q96KQ7, (Mouse) Q9Z148
Entrez Gene ID: (Human) 10919, (Mouse) 110147
p53 binding
protein binding
zinc ion binding
protein-lysine N-methyltransferase activity
histone-lysine N-methyltransferase activity
histone methyltransferase activity (H3-K9 specific)
histone methyltransferase activity (H3-K27 specific)
C2H2 zinc finger domain binding
promoter-specific chromatin binding
rRNA (adenine-N6,N6-)-dimethyltransferase activity
RNA polymerase II regulatory region sequence-specific DNA binding
protein C-terminal carboxyl O-methyltransferase activity
tRNA (guanine-N2-)-methyltransferase activity
methyltransferase activity
C-methyltransferase activity
N-methyltransferase activity
O-methyltransferase activity
S-methyltransferase activity
RNA methyltransferase activity
mRNA methyltransferase activity
tRNA methyltransferase activity
protein methyltransferase activity
2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity
rRNA methyltransferase activity
rRNA (uridine-2'-O-)-methyltransferase activity
rRNA (adenine-N6-)-methyltransferase activity
DNA-methyltransferase activity
tRNA (guanine-N1-)-methyltransferase activity
rRNA (cytosine-C5-)-methyltransferase activity
selenocysteine methyltransferase activity
arginine N-methyltransferase activity
protein-arginine N-methyltransferase activity
lysine N-methyltransferase activity
tRNA (uracil) methyltransferase activity
tRNA (guanine) methyltransferase activity
tRNA (adenine) methyltransferase activity
tRNA (cytosine) methyltransferase activity
tRNA (cytosine-5-)-methyltransferase activity
tRNA (adenine-N1-)-methyltransferase activity
rRNA (adenine) methyltransferase activity
rRNA (cytosine) methyltransferase activity
rRNA (guanine) methyltransferase activity
rRNA (uridine) methyltransferase activity
transferase activity
protein C-terminal leucine carboxyl O-methyltransferase activity
1-phenanthrol methyltransferase activity
protein-arginine N5-methyltransferase activity
methylarsonite methyltransferase activity
dimethylarsinite methyltransferase activity
4,5-dihydroxybenzo(a)pyrene methyltransferase activity
1-hydroxypyrene methyltransferase activity
1-hydroxy-6-methoxypyrene methyltransferase activity
demethylmenaquinone methyltransferase activity
cobalt-precorrin-6B C5-methyltransferase activity
cobalt-precorrin-7 C15-methyltransferase activity
cobalt-precorrin-5B C1-methyltransferase activity
cobalt-precorrin-3 C17-methyltransferase activity
dimethylamine methyltransferase activity
hydroxyneurosporene-O-methyltransferase activity
tRNA (adenine-57, 58-N(1)-) methyltransferase activity
methylamine-specific methylcobalamin:coenzyme M methyltransferase activity
trimethylamine methyltransferase activity
methanol-specific methylcobalamin:coenzyme M methyltransferase activity
monomethylamine methyltransferase activity
metal ion binding
P-methyltransferase activity
Se-methyltransferase activity
2-phytyl-1,4-naphthoquinone methyltransferase activity
tRNA (uracil-2'-O-)-methyltransferase activity
tRNA (cytosine-2'-O-)-methyltransferase activity
phosphomethylethanolamine N-methyltransferase activity
tRNA (cytosine-3-)-methyltransferase activity
rRNA (cytosine-2'-O-)-methyltransferase activity
rRNA (cytosine-N4-)-methyltransferase activity
trihydroxyferuloyl spermidine O-methyltransferase activity
negative regulation of transcription from RNA polymerase II promoter
regulation of DNA replication
DNA methylation
regulation of transcription from RNA polymerase II promoter
synaptonemal complex assembly
germ cell development
spermatid development
long-term memory
fertilization
DNA methylation on cytosine within a CG sequence
chromatin modification
histone methylation
peptidyl-lysine dimethylation
methylation
histone lysine methylation
organ growth
cellular response to drug
regulation of DNA methylation
histone H3-K9 methylation
regulation of histone H3-K4 methylation
regulation of histone H3-K9 methylation
histone H3-K27 methylation
cellular response to starvation
regulation of signal transduction by p53 class mediator
