Invitrogen

ELK3 Polyclonal Antibody

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

查看其他11个ELK3抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (1)
高级验证 (1)

图: 1 / 2

ELK3 Antibody (PA5-68978) in WB

Western blot analysis of ELK3 on mouse small intestine tissue lysate. The sample was probed with a ELK3 polyclonal antibody (Product # PA5-68978) using a primary antibody dilution of 0.2-1.0 µg/mL.

Product Image Gallery

ELK3 Antibody — Silencing ELK3 inhibited the proliferation, adhesion, colony-forming, and migration of DU145 cells in vitro. (a) DU145 cells were transfected with siRNAs for 72h, the expression of ELK3 and ACTIN were detected by western blotting (up panel); the effect of ELK3 knockdown on DU145 cell proliferation was examined by CCK-8 assay (lower panel). (b) Effect of ELK3 knockdown on cell adhesion. (c) Effect of ELK3 knockdown on cell colony-forming. (d) Transwell assay was performed to test the effect of ELK3 knockdown on cell migration ability. Cells were fixed with 4% paraformaldehyde and stained with crystal violet, then dissolved in 33% v/v acetic acid and measured the absorbance at 570 nm (c, d). Data were averaged from three parallel experiments and are given as mean±SD. *P < 0.05, **P < 0.01, vs NC-siRNA. {KD}

产品信息

PA5-68978

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.2-1.0 µg/mL

产品规格

种属反应

Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

synthetic peptide corresponding to a region of mouse ELK3 (aa 1-50).

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS with 2% sucrose

内含物

0.09% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

RRID

AB_2689057

产品详细信息

This target displays homology in the following species: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 93%; Rat: 100%; Zebrafish: 93%

靶标信息

The protein encoded by this gene is a member of the ETS-domain transcription factor family and the ternary complex factor (TCF) subfamily. Proteins in this subfamily regulate transcription when recruited by serum response factor to bind to serum response elements. This protein is activated by signal-induced phosphorylation; studies in rodents suggest that it is a transcriptional inhibitor in the absence of Ras, but activates transcription when Ras is present.

仅用于科研。不用于诊断过程。未经明确授权不得转售。