OriGene

ENG Monoclonal Antibody (OTI8A1), TrueMAB™

查看其他84个CD105 (Endoglin)抗体

抗体检测数据 (4)

ENG Antibody in Immunocytochemistry (ICC/IF)

ENG Antibody in Immunocytochemistry (ICC/IF)

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ENG Antibody (TA506170) in ICC/IF

Immunofluorescent staining of HeLa cells using anti-ENG mouse monoclonal antibody (TA506170).

ENG Antibody in Immunocytochemistry (ICC/IF)

ENG Antibody in Western Blot (WB)

ENG Antibody in Western Blot (WB)

ENG Antibody in Western Blot (WB)

Product Image Gallery

ENG Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent staining of HeLa cells using anti-ENG mouse monoclonal antibody (TA506170).

ENG Antibody in Western Blot (WB) — Western blot analysis of extracts (35 µg) from 9 different cell lines by using anti-ENG monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

ENG Antibody in Western Blot (WB) — HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ENG (RC221699, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-ENG. Positive lysates LY424919 (100 µg) and LC424919 (20 µg) can be purchased separately from OriGene.

ENG Antibody in Western Blot (WB) — Western blot analysis of extracts (10 µg) from a mouse cell line and 3 different mouse tissues by using anti-ENG monoclonal antibody. (1:200)

产品信息

TA506170

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:200-1:4000

-

免疫细胞化学 (ICC/IF)

1:100

-

产品规格

种属反应

Dog, Human, Mouse, Rat

宿主/亚型

Mouse / IgG2b

分类

Monoclonal

类型

Antibody

克隆号

OTI8A1

抗原

Full length human recombinant protein of human ENG produced in HEK293T cell.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS with 1% BSA, 50% glycerol

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

靶标信息

CD105 or Endoglin is a Type I transmembrane protein, which is highly expressed on human vascular endothelial cells. It exists on an O- and N-glycosylated homodimer. Up regulation of endoglin expression has been demonstrated in tumor vasculature and proliferating cells, suggesting that it is a proliferation associated endothelial marker. CD105 binds to TGF beta 1 and 3 with high affinity but not to TGF beta 2.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: CD105; CD105 antigen; Cell surface MJ7/18 antigen; Endoglin; endoglin (Osler-Rendu-Weber syndrome 1); ORW; transmembrane glycoprotein

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基因别名: AI528660; AI662476; CD105; Edg; END; Endo; ENG; HHT1; ORW1; S-endoglin

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UniProt ID: (Human) P17813, (Mouse) Q63961

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Entrez Gene ID: (Human) 2022, (Dog) 609166, (Mouse) 13805, (Rat) 497010

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transmembrane signaling receptor activity transforming growth factor beta receptor, cytoplasmic mediator activity type II transforming growth factor beta receptor binding protein binding galactose binding glycosaminoglycan binding type I transforming growth factor beta receptor binding BMP binding protein homodimerization activity activin binding transforming growth factor beta binding transforming growth factor beta-activated receptor activity transmembrane signal receptor

参与通路

chronological cell aging patterning of blood vessels vasculogenesis positive regulation of protein phosphorylation heart looping sprouting angiogenesis endocardial cushion morphogenesis cardiac ventricle morphogenesis cardiac atrium morphogenesis ventricular trabecula myocardium morphogenesis cell migration involved in endocardial cushion formation regulation of transcription, DNA-templated cell adhesion negative regulation of gene expression positive regulation of pathway-restricted SMAD protein phosphorylation regulation of transforming growth factor beta receptor signaling pathway central nervous system vasculogenesis negative regulation of cell migration BMP signaling pathway positive regulation of BMP signaling pathway negative regulation of protein autophosphorylation dorsal aorta morphogenesis positive regulation of angiogenesis positive regulation of transcription from RNA polymerase II promoter smooth muscle tissue development artery morphogenesis venous blood vessel morphogenesis endocardial cushion to mesenchymal transition positive regulation of epithelial to mesenchymal transition involved in endocardial cushion formation negative regulation of transcription from RNA polymerase II promoter angiogenesis response to hypoxia negative regulation of endothelial cell proliferation positive regulation of systemic arterial blood pressure protein phosphorylation transmembrane receptor protein serine/threonine kinase signaling pathway transforming growth factor beta receptor signaling pathway heart development positive regulation of gene expression cell migration extracellular matrix disassembly positive regulation of collagen biosynthetic process intracellular signal transduction wound healing cell motility negative regulation of nitric-oxide synthase activity cell chemotaxis negative regulation of pathway-restricted SMAD protein phosphorylation extracellular matrix constituent secretion detection of hypoxia response to corticosteroid response to statin bone development cellular response to mechanical stimulus response to transforming growth factor beta