Invitrogen

ERK1/ERK2 Polyclonal Antibody

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ERK1/ERK2 Antibody (PA1-4703) in WB

Western blot of ERK1/2 in rat hippocampal homogenate showing specific immunolabeling of a band at ~42-44 kDa corresponding to ERK1/ERK2 polyclonal antibody (Product # PA1-4703).

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产品信息

PA1-4703

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

查看3篇已发表文章 3篇已发表文章

免疫细胞化学 (ICC/IF)

1:250

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to the C-terminal region of rat MAPK1/2 conjugated to KLH

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

纯化类型

Affinity chromatography

保存液

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

内含物

no preservative

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2141430

产品详细信息

This antibody is predicted to react with mouse based on 100% sequence homology.

In Western blot, PA1-4703 detects the~43 kDa ERK/MAPK protein.

靶标信息

ERK1 and ERK2 are widely expressed and are involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells. Many different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors and transforming agents, activate the ERK1 and ERK2 pathways. When growth factors bind to the receptor tyrosine kinase, Ras interacts with Raf, the serine/threonine protein kinase and activates it as well. Once actived, Raf phosphorylates serine residue in 2 further kinases, MEK1/2, which in turn phosphorylates tyrosine/threonine in extracellular-signal regulated kinase (ERK) 1/2. Upon activation, the ERKs either phosphorylate a number of cytoplasmic targets or migrate to the nucleus, where they phosphorylate and activate a number of transcription factors such as c-Fos and Elk-1.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Disc1 Carrier Mice Exhibit Alterations in Neural pIGF-1Rβ and Related Kinase Expression.

Frontiers in cellular neuroscience

Sultana R,Shrestha A,Lee CC,Ogundele OM

PA1-4703 was used in Western Blotting to demonstrate that the expression of active IGF-1Rβ (pIGF-1Rβ) is altered in the hippocampus and prefrontal cortex (PFC) of disc1 mutant mice and vary with the dose of the mutation (homozygous and heterozygous).

Mon Sep 28 00:00:00 EDT 2020

Disc1 Carrier Mice Exhibit Alterations in Neural pIGF-1Rβ and Related Kinase Expression.

Frontiers in cellular neuroscience

Sultana R,Shrestha A,Lee CC,Ogundele OM

Mon Sep 28 00:00:00 EDT 2020

SK Channel Modulates Synaptic Plasticity by Tuning CaMKIIα/β Dynamics.

Frontiers in synaptic neuroscience

Shrestha A,Sultana R,Lee CC,Ogundele OM

PA1-4703 was used in Western Blotting to demonstrate that positive modulation of SK channel at hippocampal synapses likely refine GluN1-linked plasticity by tuning dendritic localization of CaMKIIα.

Tue Sep 29 00:00:00 EDT 2020

PDGF-BB Preserves Mitochondrial Morphology, Attenuates ROS Production, and Upregulates Neuroglobin in an Astrocytic Model Under Rotenone Insult.

Molecular neurobiology

Cabezas R,Vega-Vela NE,González-Sanmiguel J,González J,Esquinas P,Echeverria V,Barreto GE

PA14703 was used in western blot to discuss mechanisms associated with platelet-derived growth factor, subtype BB protection against oxidative damage

Sun Apr 01 00:00:00 EDT 2018

生物信息学

蛋白别名: ERK-1; ERK-2; erk1 erk2; ERK1b; ERT1; ERT2; Extracellular signal-regulated kinase 1; Extracellular signal-regulated kinase 2; extracellular signal-related kinase 1; extracellular-signal-regulated kinase 2; Insulin-stimulated MAP2 kinase; MAP kinase 1; MAP kinase 2; MAP kinase 3; MAP kinase isoform p42; MAP kinase isoform p44; MAPK 1; MAPK 2; MAPK 3; Microtubule-associated protein 2 kinase; mitogen activated protein kinase 1; mitogen-activated 3; Mitogen-activated protein kinase 1; Mitogen-activated protein kinase 2; Mitogen-activated protein kinase 3; MNK1; p42 MAP Kinase; p42-MAPK; p44 MAP kinase; p44-ERK1; p44-MAPK; pp42/MAP kinase; protein tyrosine kinase ERK2

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基因别名: 9030612K14Rik; AA407128; AU018647; C78273; ERK; ERK-1; ERK-2; ERK1; ERK2; ERT1; ERT2; Esrk1; HS44KDAP; HUMKER1A; Mapk; MAPK1; MAPK2; MAPK3; MNK1; Mtap2k; p38; p40; p41; p41mapk; p42-MAPK; P42MAPK; p44; p44-ERK1; p44-MAPK; P44ERK1; P44MAPK; PRKM1; PRKM2; PRKM3