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ERK2 Antibody (PA5-18329) in ICC/IF
Immunocytochemistry analysis of ERK2 using ERK2 Polyclonal Antibody (Product # PA5-18329) in paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
产品信息
PA5-18329
产品规格
种属反应
Human
宿主/亚型
Goat
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide sequence (CAAGPEMVRGQVF) corresponding to the internal amino acids of MAPK1 (aa 9-19).
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Ammonium sulfate precipitation
保存液
TBS, pH 7.3, with 0.5% BSA
内含物
0.02% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_10979280
产品详细信息
This antibody is predicted to react with bovine, canine, mouse and rat based on sequence homology.
This antibody is tested in Peptide ELISA: antibody detection limit dilution 8,000.
靶标信息
MAPK1 (ERK2) is a serine/threonine kinase that acts as an important activator of p90 RSK, MSK, ELK1, and Stat3, and is involved in the MAPK pathway. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: ERK-2; ERT1; Extracellular signal-regulated kinase 2; MAP kinase 1; MAP kinase 2; MAP kinase isoform p42; MAPK 2; Mitogen-activated protein kinase 1; Mitogen-activated protein kinase 2; p42-MAPK; protein tyrosine kinase ERK2
基因别名: ERK; ERK-2; ERK2; ERT1; MAPK1; MAPK2; p38; p40; p41; p41mapk; p42-MAPK; P42MAPK; PRKM1; PRKM2
UniProt ID: (Human) P28482
Entrez Gene ID: (Human) 5594
phosphotyrosine binding
DNA binding
protein serine/threonine kinase activity
MAP kinase activity
protein binding
ATP binding
transcription factor binding
RNA polymerase II carboxy-terminal domain kinase activity
kinase activity
phosphatase binding
mitogen-activated protein kinase kinase kinase binding
identical protein binding
non-receptor serine/threonine protein kinase
MAPK cascade
activation of MAPK activity
MAPK import into nucleus
transcription, DNA-templated
protein phosphorylation
apoptotic process
chemotaxis
response to stress
cellular response to DNA damage stimulus
cell cycle
signal transduction
chemical synaptic transmission
axon guidance
positive regulation of cell proliferation
fibroblast growth factor receptor signaling pathway
response to toxic substance
positive regulation of peptidyl-threonine phosphorylation
regulation of phosphatidylinositol 3-kinase signaling
viral process
peptidyl-serine phosphorylation
peptidyl-threonine phosphorylation
sensory perception of pain
cytosine metabolic process
platelet activation
positive regulation of cell migration
thyroid gland development
regulation of protein stability
lipopolysaccharide-mediated signaling pathway
positive regulation of telomere maintenance via telomerase
regulation of stress-activated MAPK cascade
mammary gland epithelial cell proliferation
Fc-epsilon receptor signaling pathway
Fc-gamma receptor signaling pathway involved in phagocytosis
ERBB signaling pathway
outer ear morphogenesis
response to exogenous dsRNA
response to estrogen
negative regulation of cell differentiation
positive regulation of translation
positive regulation of transcription, DNA-templated
thymus development
T cell receptor signaling pathway
B cell receptor signaling pathway
regulation of sequence-specific DNA binding transcription factor activity
regulation of cytoskeleton organization
positive regulation of telomerase activity
Bergmann glial cell differentiation
long-term synaptic potentiation
face development
JAK-STAT cascade involved in growth hormone signaling pathway
lung morphogenesis
trachea formation
labyrinthine layer blood vessel development
cardiac neural crest cell development involved in heart development
ERK1 and ERK2 cascade
response to epidermal growth factor
caveolin-mediated endocytosis
regulation of Golgi inheritance
cellular response to granulocyte macrophage colony-stimulating factor stimulus
regulation of cellular response to heat
positive regulation of telomere capping
regulation of early endosome to late endosome transport
