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ERK3 Antibody (MA1-101) in ICC/IF
Immunofluorescent analysis of ERK3 (green) showing positive staining in the cytoplasm and nucleus of NIH-3T3 cells (right) compared with a negative control in the absence of primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature and probed with an ERK3 monoclonal antibody (Product # MA1-101) in 3% BSA-PBS at a dilution of 1:200 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated... View More
产品信息
MA1-101
产品规格
种属反应
Human,
Mouse
宿主/亚型
Mouse
/ IgG
分类
Monoclonal
类型
Antibody
克隆号
5E1
抗原
Recombinant protein expressed in E.coli corresponding to amino acids 345-721 of human ERK3
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS with 1mg/mL BSA, 30% glycerol
内含物
0.05% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2536749
产品详细信息
MA1-101 has been successfully used in Western blot, immunohistochemistry and immunfluorescence applications on human and mouse samples.
靶标信息
The protein encoded by this gene is a member of the Ser/Thr protein kinase family, and is most closely related to mitogen-activated protein kinases (MAP kinases). MAP kinases also known as extracellular signal-regulated kinases (ERKs), are activated through protein phosphorylation cascades and act as integration points for multiple biochemical signals. This kinase is localized in the nucleus, and has been reported to be activated in fibroblasts upon treatment with serum or phorbol esters. Tissue specificity: Highest expression in the skeletal muscle, followed by the brain. Also found in heart, placenta, lung, liver, pancreas, kidney and skin fibroblasts.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: DKFZp686F03189; ERK-3; Erk-3 related; Extracellular signal-regulated kinase 3; extracellular signal-regulated kinase, p97; MAP kinase 6; MAP kinase isoform p97; MAPK 6; mitogen activated protein kinase 4; mitogen activated protein kinase 6; Mitogen-activated protein kinase 6; p97-MAPK; protein kinase, mitogen activated kinase 4; protein kinase, mitogen-activated 5; protein kinase, mitogen-activated 6
基因别名: 2610021I23Rik; D130053K17Rik; ERK3; HsT17250; Mapk4; MAPK6; Mapk63; p97MAPK; Prkm4; PRKM6
UniProt ID: (Human) Q16659, (Mouse) Q61532
Entrez Gene ID: (Human) 5597, (Mouse) 50772
protein serine/threonine kinase activity
MAP kinase activity
protein binding
ATP binding
protein kinase binding
protein heterodimerization activity
nucleotide binding
protein kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
non-receptor serine/threonine protein kinase
