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ERp57 Antibody (MA5-27689) in ICC/IF
Immunofluorescent analysis of ERp57 in human HeLa cells (human cervical cancer). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with ERp57 monoclonal antibody (Product # MA5-27689) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Mouse 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:200, 1:1000 (60 min at RT) and 1:5000 (5 min at RT). Images are shown as follows: (A) DAPI (blue) nuclear stain, (B) Phalloidin Texas Red F-Actin stain, (C) ERp57 Antibody, (D) Merged image. Magnifica... View More
产品信息
MA5-27689
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
4F9
抗原
Human recombinant ERp57 (Grp58) protein corresponding to amino acids 25 - 505 (his-tagged)
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein G
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.09% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2735177
产品详细信息
A 1:1,000 dilution of MA5-27689 was sufficient for detection of ERp57 in 15 µg of HeLa by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.|Detects approximately 57 kDa protein.
靶标信息
This gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 58 kDa glucose-regulated protein; 58 kDa microsomal protein; Disulfide isomerase ER-60; endoplasmic reticulum P58; Endoplasmic reticulum resident protein 57; Endoplasmic reticulum resident protein 60; epididymis secretory protein Li 269; epididymis secretory sperm binding protein Li 93n; ER p57; ER protein 57; ER protein 60; ERp 57; glucose regulated protein, 58kDa; GRP 57; GRP 58; p58; PDIA 3; phospholipase C-alpha; PI PLC; protein disulfide isomerase family A, member 3; protein disulfide isomerase-associated 3; Protein disulfide-isomerase A3
基因别名: ER60; ERP57; ERP60; ERp61; GRP57; GRP58; HEL-S-269; HEL-S-93n; HsT17083; P58; PDIA3; PI-PLC
UniProt ID: (Human) P30101
Entrez Gene ID: (Human) 2923
antigen processing and presentation of peptide antigen via MHC class I
protein folding
proteolysis
protein import into nucleus
protein retention in ER lumen
signal transduction
protein folding in endoplasmic reticulum
response to endoplasmic reticulum stress
cell redox homeostasis
oxidation-reduction process
positive regulation of extrinsic apoptotic signaling pathway
