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ETS2 Antibody (PA5-28053) in ICC/IF
Immunofluorescence analysis of ETS2 was performed using HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with ETS2 Polyclonal Antibody (Product # PA5-28053) at 1:200 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 (Product # A27034, 1:2000 dilution) for 45 mi... View More
产品信息
PA5-28053
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Rat
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fragment corresponding to a region within amino acids 1 and 287 of Human ETS2
偶联物
形式
Liquid
浓度
0.35 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7, with 20% glycerol, 1% BSA
内含物
0.025% ProClin 300
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2545529
产品详细信息
Recommended positive controls: HeLa.
Predicted reactivity: Mouse (87%), Rat (88%), Bovine (89%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
ETS transcriptions factors, such as ETS2, regulate numerous genes and are involved in stem cell development, cell senescence and death, and tumorigenesis. The conserved ETS domain within these proteins is a winged helix-turn-helix DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T of target genes.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: avian erythroblastosis virus E26 (v-ets) oncogene homolog 2; E26 avian leukemia oncogene 2, 3' domain; oncogene ETS-2; Protein C-ets-2; v-ets avian erythroblastosis virus E2 oncogene homolog 2; v-ets avian erythroblastosis virus E26 oncogene homolog 2; v-ets erythroblastosis virus E26 oncogene homolog 2
基因别名: ETS2; ETS2IT1
UniProt ID: (Human) A6NM68
Entrez Gene ID: (Human) 2114, (Rat) 304063
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
glucocorticoid receptor binding
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
sequence-specific DNA binding
winged helix/forkhead transcription factor
negative regulation of transcription from RNA polymerase II promoter
skeletal system development
ectodermal cell fate commitment
transcription, DNA-templated
mesoderm development
cell differentiation
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
primitive streak formation
transcription from RNA polymerase II promoter
