Invitrogen

EZH2 Polyclonal Antibody

EZH2 Antibody in ChIP-Sequencing (ChIP-seq)

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EZH2 Antibody (49-1043) in ChIP-seq

ChIP was performed on sheared chromatin from 4 million K562 cells using 2 µg of the anti-EZH2 antibody (Product # 49-1043). The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. The figure shows the peak distribution along the short arm and a 6 Mb region containing several enriched regions of human chromosome 3 (figure A and B, respectively), and ... View More

EZH2 Antibody in Immunocytochemistry (ICC/IF)

EZH2 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

EZH2 Antibody in Immunohistochemistry, Immunohistochemistry (Paraffin) (IHC, IHC (P))

Product Image Gallery

EZH2 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. PC-3 cells were transfected with EZH2 siRNA and decrease in signal intensity was observed in Western Blot application using Anti-EZH2 Polyclonal Antibody (Product # 49-1043). {KD}

产品信息

49-1043

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

查看4篇已发表文章 4篇已发表文章

免疫组化 (IHC)

查看3篇已发表文章 3篇已发表文章

免疫组化（石蜡） (IHC (P))

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

1:1,000

查看1篇已发表文章 1篇已发表文章

染色质免疫沉淀实验 (ChIP)

1-10 µg/4x10^6 cells

查看4篇已发表文章 4篇已发表文章

染色质免疫沉淀测序 (ChIP-Seq)

2 µg/4x10^6 cells

产品规格

种属反应

Human, Mouse

已发表种属

Human, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

N-terminus (aa1-343) of the mouse Ezh2 protein

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS

内含物

0.05% ProClin 300, 0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Dry ice

RRID

AB_2533892

靶标信息

EZH2 (Histone-lysine N-methyltransferase) is a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. EZH2 may play a role in the hematopoietic and central nervous systems. Mutations affecting EZH2 can cause Weaver syndrome (WVS).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Hypoxic condition induced H3K27me3 modification of the LncRNA Tmem235 promoter thus supporting apoptosis of BMSCs.

Apoptosis : an international journal on programmed cell death

Zhang F,Luo H,Peng W,Wang L,Wang T,Xie Z,Zhang J,Dong W,Zheng X,Liu G,Zhu X,Kang Q,Tian X

Published figure using EZH2 polyclonal antibody (Product # 49-1043) in Western Blot

Sat Oct 01 00:00:00 UTC 2022

Hypoxic condition induced H3K27me3 modification of the LncRNA Tmem235 promoter thus supporting apoptosis of BMSCs.

Apoptosis : an international journal on programmed cell death

Zhang F,Luo H,Peng W,Wang L,Wang T,Xie Z,Zhang J,Dong W,Zheng X,Liu G,Zhu X,Kang Q,Tian X

Published figure using EZH2 polyclonal antibody (Product # 49-1043) in Western Blot

Sat Oct 01 00:00:00 UTC 2022

Demethylation of H3K9 and H3K27 Contributes to the Tubular Renal Damage Triggered by Endoplasmic Reticulum Stress.

Antioxidants (Basel, Switzerland)

Diaz-Bulnes P,Saiz ML,Corte-Iglesias V,Rodrigues-Diez RR,Bernardo Florez A,Ruiz Bernet C,Martin Martin C,Ruiz-Ortega M,Suarez-Alvarez B,López-Larrea C

49-1043 was used in Chromatin immunoprecipitation to investigate whether epigenetic dynamics mediated by histone H3K9 and H3K27 methylation might help to reduce or inhibit the exacerbated and maladaptive UPR triggered in tubular epithelial cells.

Tue Jul 12 00:00:00 UTC 2022

HOPX regulates bone marrow-derived mesenchymal stromal cell fate determination via suppression of adipogenic gene pathways.

Scientific reports

Hng CH,Camp E,Anderson P,Breen J,Zannettino A,Gronthos S

49-1043 was used in Chromatin immunoprecipitation to study the role of homeodomain-only protein homeobox (HOPX) gene expression during bone marrow stromal cell (BMSC) growth and differentiation.

Thu Jul 09 00:00:00 UTC 2020

HOPX regulates bone marrow-derived mesenchymal stromal cell fate determination via suppression of adipogenic gene pathways.

Scientific reports

Hng CH,Camp E,Anderson P,Breen J,Zannettino A,Gronthos S

49-1043 was used in Chromatin immunoprecipitation to study the role of homeodomain-only protein homeobox (HOPX) gene expression during bone marrow stromal cell (BMSC) growth and differentiation.

Thu Jul 09 00:00:00 UTC 2020

Methylation and PTEN activation in dental pulp mesenchymal stem cells promotes osteogenesis and reduces oncogenesis.

Nature communications

Shen WC,Lai YC,Li LH,Liao K,Lai HC,Kao SY,Wang J,Chuong CM,Hung SC

49-1043 was used in Western Blotting to demonstrate how several epigenetic factors broadly affect lineage commitment and tumorigenesis, which should be considered when developing therapeutic uses of stem cells.

Mon May 20 00:00:00 UTC 2019

CCN3/NOV gene expression in human prostate cancer is directly suppressed by the androgen receptor.

Oncogene

Wu L,Runkle C,Jin HJ,Yu J,Li J,Yang X,Kuzel T,Lee C,Yu J

49-1043 was used in ChIP assay to report a tumor-suppressive role of NOV in prostate cancer.

Thu Jan 23 00:00:00 UTC 2014

The central role of EED in the orchestration of polycomb group complexes.

Nature communications

Cao Q,Wang X,Zhao M,Yang R,Malik R,Qiao Y,Poliakov A,Yocum AK,Li Y,Chen W,Cao X,Jiang X,Dahiya A,Harris C,Feng FY,Kalantry S,Qin ZS,Dhanasekaran SM,Chinnaiyan AM

49-1043 was used in ChIP assay and western blot to study the Involvement of EED in the organization of polycomb group complexes

Tue Oct 27 00:00:00 UTC 2015

The central role of EED in the orchestration of polycomb group complexes.

Nature communications

Cao Q,Wang X,Zhao M,Yang R,Malik R,Qiao Y,Poliakov A,Yocum AK,Li Y,Chen W,Cao X,Jiang X,Dahiya A,Harris C,Feng FY,Kalantry S,Qin ZS,Dhanasekaran SM,Chinnaiyan AM

49-1043 was used in ChIP assay and western blot to study the Involvement of EED in the organization of polycomb group complexes

Tue Oct 27 00:00:00 UTC 2015

Histone-modifying genes as biomarkers in hepatocellular carcinoma.

International journal of clinical and experimental pathology

Hung SY,Lin HH,Yeh KT,Chang JG

Published figure using EZH2 polyclonal antibody (Product # 49-1043) in Immunohistochemistry

Mon Feb 23 00:00:00 UTC 2015

Histone-modifying genes as biomarkers in hepatocellular carcinoma.

International journal of clinical and experimental pathology

Hung SY,Lin HH,Yeh KT,Chang JG

Published figure using EZH2 polyclonal antibody (Product # 49-1043) in Immunohistochemistry

Mon Feb 23 00:00:00 UTC 2015

EZH2 regulates expression of p57 and contributes to progression of ovarian cancer in vitro and in vivo.

Cancer science

Guo J,Cai J,Yu L,Tang H,Chen C,Wang Z

Tue Mar 01 00:00:00 UTC 2011

Enhancer of Zeste homolog 2 (EZH2) is overexpressed in recurrent nasopharyngeal carcinoma and is regulated by miR-26a, miR-101, and miR-98.

Cell death & disease

Alajez NM,Shi W,Hui AB,Bruce J,Lenarduzzi M,Ito E,Yue S,O'Sullivan B,Liu FF

Published figure using EZH2 polyclonal antibody (Product # 49-1043) in Immunofluorescence

Thu Oct 21 00:00:00 UTC 2010

Enhancer of Zeste homolog 2 (EZH2) is overexpressed in recurrent nasopharyngeal carcinoma and is regulated by miR-26a, miR-101, and miR-98.

Cell death & disease

Alajez NM,Shi W,Hui AB,Bruce J,Lenarduzzi M,Ito E,Yue S,O'Sullivan B,Liu FF

Published figure using EZH2 polyclonal antibody (Product # 49-1043) in Immunofluorescence

Thu Oct 21 00:00:00 UTC 2010

Enhancer of Zeste homolog 2 (EZH2) is overexpressed in recurrent nasopharyngeal carcinoma and is regulated by miR-26a, miR-101, and miR-98.

Cell death & disease

Alajez NM,Shi W,Hui AB,Bruce J,Lenarduzzi M,Ito E,Yue S,O'Sullivan B,Liu FF

Published figure using EZH2 polyclonal antibody (Product # 49-1043) in Immunofluorescence

Thu Oct 21 00:00:00 UTC 2010

生物信息学

蛋白别名: an; Enhancer of zeste homolog 2; ENX 1; ENX-1; EZH 2; EZH-2; Histone-lysine N-methyltransferase EZH2; Lysine N-methyltransferase 6; OTTHUMP00000213774; OTTHUMP00000213778; OTTHUMP00000213779; OTTHUMP00000213780

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基因别名: ENX-1; ENX1; Enx1h; EZH1; EZH2; EZH2b; KMT6; KMT6A; mKIAA4065; WVS; WVS2

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UniProt ID: (Human) Q15910, (Mouse) Q61188

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Entrez Gene ID: (Human) 2146, (Mouse) 14056

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功能

core promoter binding DNA binding chromatin binding RNA binding protein binding protein-lysine N-methyltransferase activity histone-lysine N-methyltransferase activity chromatin DNA binding histone methyltransferase activity ribonucleoprotein complex binding sequence-specific DNA binding histone methyltransferase activity (H3-K27 specific) promoter-specific chromatin binding regulatory region DNA binding methyltransferase activity transferase activity histone modifying enzyme

参与通路

EZH2 Polyclonal Antibody

EZH2 Antibody (49-1043) in ChIP-seq

产品信息

49-1043

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（石蜡） (IHC (P))

免疫细胞化学 (ICC/IF)

染色质免疫沉淀实验 (ChIP)

染色质免疫沉淀测序 (ChIP-Seq)

种属反应

已发表种属

宿主/亚型

分类

类型

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

EZH2 Polyclonal Antibody

EZH2 Antibody (49-1043) in ChIP-seq

产品信息

49-1043

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（石蜡） (IHC (P))

免疫细胞化学 (ICC/IF)

染色质免疫沉淀 实验 (ChIP)

染色质免疫沉淀测序 (ChIP-Seq)

种属反应

已发表种属

宿主/亚型

分类

类型

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

染色质免疫沉淀实验 (ChIP)