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Invitrogen
EZH2 Monoclonal Antibody (144CT2.1.1.5)
高级验证
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
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EZH2 Antibody (MA5-18108) in ICC/IF
Immunofluorescence analysis of EZH2 was performed using 70% confluent log phase HCT 116 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with EZH2 Mouse Monoclonal Antibody (144CT2.1.1.5) (Product # MA5-18108) at 1: 50 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Pr... View More
产品信息
MA5-18108
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
144CT2.1.1.5
抗原
EZH2 recombinant protein
偶联物
形式
Liquid
浓度
0.48 mg/mL
纯化类型
Protein G
保存液
PBS, pH 7.4
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2539482
产品详细信息
This antibody is predicted to react with non-human primate based on sequence homology.
靶标信息
EZH2 (Histone-lysine N-methyltransferase) is a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. EZH2 may play a role in the hematopoietic and central nervous systems. Mutations affecting EZH2 can cause Weaver syndrome (WVS).
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: an; Enhancer of zeste homolog 2; ENX 1; ENX-1; EZH 2; EZH-2; Histone-lysine N-methyltransferase EZH2; Lysine N-methyltransferase 6; OTTHUMP00000213774; OTTHUMP00000213778; OTTHUMP00000213779; OTTHUMP00000213780
基因别名: ENX-1; ENX1; EZH1; EZH2; EZH2b; KMT6; KMT6A; WVS; WVS2
UniProt ID: (Human) Q15910
Entrez Gene ID: (Human) 2146
core promoter binding
DNA binding
chromatin binding
RNA binding
protein binding
protein-lysine N-methyltransferase activity
histone-lysine N-methyltransferase activity
chromatin DNA binding
histone methyltransferase activity
ribonucleoprotein complex binding
sequence-specific DNA binding
histone methyltransferase activity (H3-K27 specific)
promoter-specific chromatin binding
histone modifying enzyme
negative regulation of transcription from RNA polymerase II promoter
DNA methylation
chromatin organization
transcription, DNA-templated
regulation of transcription, DNA-templated
positive regulation of epithelial to mesenchymal transition
regulation of gliogenesis
skeletal muscle satellite cell maintenance involved in skeletal muscle regeneration
cerebellar cortex development
hippocampus development
response to estradiol
negative regulation of transcription elongation from RNA polymerase II promoter
regulation of cell proliferation
regulation of circadian rhythm
positive regulation of MAP kinase activity
negative regulation of sequence-specific DNA binding transcription factor activity
positive regulation of GTPase activity
negative regulation of epidermal cell differentiation
negative regulation of gene expression, epigenetic
negative regulation of transcription, DNA-templated
negative regulation of retinoic acid receptor signaling pathway
rhythmic process
negative regulation of striated muscle cell differentiation
cellular response to hydrogen peroxide
G1 to G0 transition
histone H3-K27 methylation
protein localization to chromatin
positive regulation of protein serine/threonine kinase activity
positive regulation of dendrite development
negative regulation of G1/S transition of mitotic cell cycle
