Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
Estrogen Receptor beta Monoclonal Antibody (MC10), eBioscience™
图: 1 / 5
Estrogen Receptor beta Antibody (14-9336-82) in IHC (P)
Immunohistochemistry of formalin-fixed paraffin embedded human infiltrating ductal carcinoma tissue using 30 µg/mL Mouse IgG2a K Isotype Control (left) or 30 µg/mL Anti-Human Estrogen Receptor beta Purified (right) followed by Anti-Mouse Biotin, Streptavidin-HRP, and DAB visualization.Nuclei are counterstained with hematoxylin.
产品信息
14-9336-82
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human
宿主/亚型
Mouse
/ IgG2a, kappa
分类
Monoclonal
类型
Antibody
克隆号
MC10
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2572943
产品详细信息
Description: The MC10 monoclonal antibody recognizes all forms of human estrogen receptor (ER) beta. ER beta is a member of a superfamily of nuclear receptors, which are ligand-dependent transcription factors. ER beta like ER alpha, becomes activated by binding the hormone estrogen, causing a dissociation from chaperone proteins and subsequent dimerization with either ER beta or ER alpha. Unlike ER alpha, the result of ER beta signaling is thought to be anti-proliferative and proapoptotic. The MC10 clone recognizes full length ER beta as well as each of its splice variants.
Applications Reported: This MC10 antibody has been reported for use in immunoprecipitation, western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, ELISA, and immunocytochemistry.
Applications Tested: This MC10 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded human tissue using either low or high pH antigen retrieval and can be used at less than or equal to 30 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
Estrogen Receptor beta (ER beta, NR3A2) protein is approximately 55kD, though multiple isoforms ranging from 36-59kD have been described. Human ER beta was initially cloned and characterized from testis. Estrogen Receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptors. The estrogen receptor is a ligand-activated transcription factor, that when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to estrogen response elements (ERE) in DNA. When bound to EREs, ER can positively or negatively regulate gene transcription through the recruitment of coactivator or corepressor proteins. There are two different forms of the estrogen receptor, alpha and beta, encoded by separate genes (ESR1 and ESR2, respectively). Estrogen receptor beta (ER beta) binds estrogens with an affinity similar to that of estrogen receptor alpha. Some ER beta isoforms dominantly inhibit the activity of estrogen receptor alpha in reproductive tissues. ER beta is found widely in many tissues throughout the body and can act as a potent tumor suppressor, playing a crucial role in many cancer types such as prostate cancer, autism spectrum disorder, medullary thyroid carcinoma, De Quervain Disease and risk of cardiovascular disease.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: ER beta; ER-beta; estrogen receptor 2 (ER beta); Estrogen receptor beta; estrogen receptor beta 4; Nuclear receptor subfamily 3 group A member 2
基因别名: ER-BETA; Erb; ESR-BETA; ESR2; ESRB; ESTRB; NR3A2
UniProt ID: (Human) Q92731
Entrez Gene ID: (Human) 2100
core promoter sequence-specific DNA binding
DNA binding
transcription factor activity, sequence-specific DNA binding
steroid hormone receptor activity
transcription coactivator activity
RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding
steroid binding
protein binding
drug binding
zinc ion binding
enzyme binding
estrogen receptor activity
estrogen response element binding
RNA polymerase II transcription factor activity, estrogen-activated sequence-specific DNA binding
peroxisome proliferator activated receptor binding
receptor antagonist activity
estradiol binding
steroid hormone binding
negative regulation of transcription from RNA polymerase II promoter
ovarian follicle development
behavioral fear response
regulation of transcription, DNA-templated
transcription initiation from RNA polymerase II promoter
signal transduction
cell-cell signaling
learning or memory
response to water deprivation
response to activity
response to insecticide
cerebellum development
amygdala development
hypothalamus development
negative regulation of cell growth
intracellular estrogen receptor signaling pathway
prostate gland development
response to nutrient levels
response to testosterone
response to genistein
cellular response to drug
positive regulation of apoptotic process
regulation of neuron apoptotic process
response to estrogen
response to human chorionic gonadotropin
estrous cycle
response to ethanol
positive regulation of epidermal growth factor receptor signaling pathway
positive regulation of transcription, DNA-templated
positive regulation of vasodilation
positive regulation of transcription from RNA polymerase II promoter
negative regulation of smooth muscle cell proliferation
negative regulation of epithelial cell proliferation
positive regulation of sequence-specific DNA binding transcription factor activity
Sertoli cell development
Sertoli cell proliferation
positive regulation of ERK1 and ERK2 cascade
cellular response to magnetism
cellular response to estradiol stimulus
response to dexamethasone
extracellular negative regulation of signal transduction
negative regulation of neuron death
response to salt
response to bisphenol A
negative regulation of feeding behavior
negative regulation of reactive oxygen species metabolic process
