Invitrogen

F4/80 Monoclonal Antibody (BM8), Pacific Orange™

产品信息

MF48030

应用

建议稀释比

已发表文章

免疫组化 (IHC)

Assay-dependent

免疫组化（冰冻） (IHC (F))

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流式细胞分析 (Flow)

Assay-dependent

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其他PubMed (Misc)

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产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

BM8

抗原

mouse F4/80 which is a macrophage-restricted cell surface glycoprotein.

偶联物

Pacific Orange™ Pacific Orange™ Pacific Orange™

激发/发射光谱

400/551 nm 查看光谱

形式

Liquid

浓度

0.3 mg/mL

纯化类型

purified

保存液

PBS with 4mg/mL BSA

内含物

0.1% sodium azide

保存条件

4° C, store in dark

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2539704

靶标信息

F4/80 antigen is a 160 kDa cell surface glycoprotein that is a member of the EGF TM7 family of proteins and shares 68% overall amino acid identity with human EGF module containing mucin like hormone receptor 1 (EMR1). Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

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Product References

TIM-3 regulates innate immune cells to induce fetomaternal tolerance.

Journal of immunology (Baltimore, Md. : 1950)

Chabtini L,Mfarrej B,Mounayar M,Zhu B,Batal I,Dakle PJ,Smith BD,Boenisch O,Najafian N,Akiba H,Yagita H,Guleria I

MF48030 was used in flow cytometry to determine the role of TIM-3-expressing innate immune cells in the regulation of tolerance at the fetomaternal interface.

Tue Jan 01 00:00:00 EST 2013

IL-34 is a tissue-restricted ligand of CSF1R required for the development of Langerhans cells and microglia.

Nature immunology

Wang Y,Szretter KJ,Vermi W,Gilfillan S,Rossini C,Cella M,Barrow AD,Diamond MS,Colonna M

MF48030 was used in flow cytometry to report that IL-34 regulates differentiation of myeloid cells in the skin epidermis and CNS.

Sun Jun 24 00:00:00 EDT 2012

CD28 promotes CD4+ T cell clonal expansion during infection independently of its YMNM and PYAP motifs.

Journal of immunology (Baltimore, Md. : 1950)

Pagán AJ,Pepper M,Chu HH,Green JM,Jenkins MK

MF48030 was used in flow cytometry to investigate which portions of CD28 are needed for T cells.

Sat Sep 15 00:00:00 EDT 2012

Resistance and augmentation of innate immunity in mice exposed to starvation.

Cellular immunology

Shen J,Ren H,Tomiyama-Miyaji C,Watanabe M,Kainuma E,Inoue M,Kuwano Y,Abo T

MF48030 was used in flow cytometry to examine immune cell population 3 days after starvation.

Tue Sep 08 00:00:00 EDT 2009

Helminth-modified pulmonary immune response protects mice from allergen-induced airway hyperresponsiveness.

Journal of immunology (Baltimore, Md. : 1950)

Mangan NE,van Rooijen N,McKenzie AN,Fallon PG

MF48030 was used in flow cytometry to elucidate the mechanisms that regulate the inverse relationship between helminth infections and allergies

Sun Jan 01 00:00:00 EST 2006

Effect of thermal injury on splenic myelopoiesis.

Shock (Augusta, Ga.)

Noel JG,Guo X,Wells-Byrum D,Schwemberger S,Caldwell CC,Ogle CK

MF48030 was used in flow cytometry to measure the number of splenic myeloid progenitors and their progeny after burn injury

Tue Feb 01 00:00:00 EST 2005

A monoclonal antibody against an antigen present on mouse macrophages and absent from monocytes.

Cell and tissue research

Malorny U,Michels E,Sorg C

MF48030 was used in immunohistochemistry - frozen section to generate and characterize a monoclonal antibody raised in the rat against cultured mouse bone marrow-derived macrophages

Tue Apr 22 00:00:00 EST 1986

生物信息学

蛋白别名: Adhesion G protein-coupled receptor E1; Cell surface glycoprotein F4/80; EGF-like module containing, mucin-like, hormone receptor-like sequence 1; EGF-like module receptor 1; EGF-like module-containing mucin-like hormone receptor-like 1; EMR1 hormone receptor; lymphocyte antigen 71

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基因别名: Adgre1; DD7A5-7; EGF-TM7; Emr1; F4/80; Gpf480; Ly71; TM7LN3

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UniProt ID: (Mouse) Q61549

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Entrez Gene ID: (Mouse) 13733

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功能

signal transducer activity transmembrane signaling receptor activity G-protein coupled receptor activity calcium ion binding G-protein coupled receptor

参与通路

signal transduction cell surface receptor signaling pathway G-protein coupled receptor signaling pathway