Invitrogen

FASN Recombinant Rabbit Monoclonal Antibody (HL2160)

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抗体检测数据 (6)

FASN Antibody in Immunocytochemistry (ICC/IF)

FASN Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 6

FASN Antibody (MA5-50408) in ICC/IF

Immunocytochemical analysis of FASN using FASN Recombinant Monoclonal Antibody (Product # MA5-50408) in Mock and differentiated 3T3-L1 cells at a dilution of 1:500. Cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Fatty Acid Synthase stained by Fatty Acid Synthase antibody (MA5-50408) diluted at 1:500. Blue: Fluoroshield with DAPI.

FASN Antibody in Immunocytochemistry (ICC/IF)

FASN Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FASN Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FASN Antibody in Western Blot (WB)

FASN Antibody in Western Blot (WB)

FASN Antibody in Western Blot (WB)

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FASN Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemical analysis of FASN using FASN Recombinant Monoclonal Antibody (Product # MA5-50408) in Mock and differentiated 3T3-L1 cells at a dilution of 1:500. Cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Fatty Acid Synthase stained by Fatty Acid Synthase antibody (MA5-50408) diluted at 1:500. Blue: Fluoroshield with DAPI.

FASN Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of FASN using FASN Recombinant Monoclonal Antibody (Product # MA5-50408) in Paraffin embedded mouse white adipocyte tissue at a dilution of 1:200. For Antigen retrieval citrate buffer, pH 6.0, as used for 15 min.

FASN Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of FASN using FASN Recombinant Monoclonal Antibody (Product # MA5-50408) in Paraffin embedded rat breast tissue at a dilution of 1:200. For Antigen retrieval citrate buffer, pH 6.0, as used for 15 min.

FASN Antibody in Western Blot (WB) — Western Blot analysis of FASN using FASN Recombinant Monoclonal Antibody (Product # MA5-50408) on 293T (30 μg), A431 (30 μg), HeLa (30 μg), and HepG2 (30 μg) whole cell extracts at a dilution of 1:5,000. Extracts were separated by 5% SDS-PAGE. An HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

FASN Antibody in Western Blot (WB) — Western Blot analysis of FASN using FASN Recombinant Monoclonal Antibody (Product # MA5-50408) in Mouse liver tissue extract (50 μg) using a dilution of 1:1,000. An HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

FASN Antibody in Western Blot (WB) — Western Blot analysis of FASN using FASN Recombinant Monoclonal Antibody (Product # MA5-50408) in 3T3-L1 (30 μg) using a dilution of 1:5,000. An HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

产品信息

MA5-50408

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-dependent

-

免疫组化（石蜡） (IHC (P))

Assay-dependent

-

免疫细胞化学 (ICC/IF)

Assay-dependent

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

HL2160

抗原

Recombinant fragment of human Fatty Acid Synthase.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS

内含物

no preservative

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_3094215

靶标信息

Fatty acid synthase is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Fatty acid synthase; MGC14367; MGC15706; short chain dehydrogenase/reductase family 27X, member 1; Type I FAS; Type I Fatty Acid Synthase

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基因别名: A630082H08Rik; FAS; FASN; OA-519; SDR27X1

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UniProt ID: (Human) P49327, (Mouse) P19096, (Rat) P12785

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Entrez Gene ID: (Human) 2194, (Mouse) 14104, (Rat) 50671

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fatty acid synthase activity [acyl-carrier-protein] S-acetyltransferase activity [acyl-carrier-protein] S-malonyltransferase activity 3-oxoacyl-[acyl-carrier-protein] synthase activity 3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase activity enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) activity oleoyl-[acyl-carrier-protein] hydrolase activity protein binding drug binding myristoyl-[acyl-carrier-protein] hydrolase activity palmitoyl-[acyl-carrier-protein] hydrolase activity phosphopantetheine binding protein homodimerization activity poly(A) RNA binding enoyl-[acyl-carrier-protein] reductase (NADPH, A-specific) activity 3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase activity NADPH binding cadherin binding involved in cell-cell adhesion 3-oxo-pimeloyl-[acp] methyl ester reductase activity catalytic activity zinc ion binding acyl-[acyl-carrier-protein] hydrolase activity oxidoreductase activity transferase activity hydrolase activity hydrolase activity, acting on ester bonds lyase activity 3-hydroxyacyl-[acyl-carrier-protein] dehydratase activity identical protein binding

参与通路

osteoblast differentiation acetyl-CoA metabolic process fatty acid metabolic process fatty acid biosynthetic process pantothenate metabolic process mammary gland development positive regulation of cellular metabolic process long-chain fatty-acyl-CoA biosynthetic process oxidation-reduction process cellular response to interleukin-4 cell-cell adhesion lipid metabolic process metabolic process biosynthetic process lipid biosynthetic process