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FGF1 Antibody (500-P17-1MG) in WB
Western Blot: To detect Human FGF-acidic by Western Blot analysis FGF1 Polyclonal Antibody (Product # 500-P17-1MG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human FGF-acidic is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
产品信息
500-P17-1MG
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Mouse
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived Recombinant Human FGF-acidic
偶联物
形式
Lyophilized
浓度
0.1-1.0 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS
内含物
no preservative
保存条件
-20°C
运输条件
Ambient
RRID
AB_2929622
产品详细信息
AA Sequence of recombinant protein: MFNLPPGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SVGEVYIKST ETGQYLAMDT DGLLYGSQTP NEECLFLERL EENHYNTYIS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human FGF-acidic. Anti-Human FGF-acidic-specific antibody was purified by affinity chromatography employing an immobilized Human FGF-acidic matrix.
Sandwich ELISA: To detect Human FGF-acidic by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human FGF-acidic (500-P17Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human FGF-acidic.
Western Blot: To detect Human FGF-acidic by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human FGF-acidic is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
靶标信息
FGF1 is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Acidic fibroblast growth factor; aFGF; beta-endothelial cell growth factor; ECGF; Endothelial cell growth factor; endothelial cell growth factor, alpha; endothelial cell growth factor, beta; FGF; FGF-1; Fibroblast growth factor; Fibroblast growth factor 1; fibroblast growth factor 1 (acidic); H-FGF-a; HBGF-1; Heparin-binding growth factor 1; M-FGF-a
基因别名: AFGF; ECGF; ECGF-beta; ECGFA; ECGFB; FGF-1; FGF-alpha; FGF1; FGFA; GLIO703; HBGF-1; HBGF1
UniProt ID: (Human) P05230
Entrez Gene ID: (Human) 2246
protein tyrosine kinase activity
Ras guanyl-nucleotide exchange factor activity
fibroblast growth factor receptor binding
integrin binding
protein binding
growth factor activity
heparin binding
1-phosphatidylinositol-3-kinase activity
Hsp70 protein binding
S100 protein binding
phosphatidylinositol-4,5-bisphosphate 3-kinase activity
growth factor
intercellular signal molecule
MAPK cascade
activation of MAPK activity
angiogenesis
organ induction
signal transduction
multicellular organism development
cell proliferation
positive regulation of cell proliferation
fibroblast growth factor receptor signaling pathway
anatomical structure morphogenesis
positive regulation of endothelial cell migration
regulation of phosphatidylinositol 3-kinase signaling
peptidyl-tyrosine phosphorylation
cell differentiation
lung development
positive regulation of cell migration
activation of protein kinase B activity
cellular response to heat
phosphatidylinositol-3-phosphate biosynthetic process
positive regulation of MAP kinase activity
positive regulation of GTPase activity
positive regulation of cholesterol biosynthetic process
positive regulation of angiogenesis
positive regulation of transcription from RNA polymerase II promoter
phosphatidylinositol phosphorylation
phosphatidylinositol-mediated signaling
positive regulation of epithelial cell proliferation
positive regulation of cell division
branch elongation involved in ureteric bud branching
positive regulation of ERK1 and ERK2 cascade
mesonephric epithelium development
regulation of endothelial tube morphogenesis
positive regulation of intracellular signal transduction
positive regulation of sprouting angiogenesis
regulation of endothelial cell chemotaxis to fibroblast growth factor
