Invitrogen

FGF17 Polyclonal Antibody, PeproTech®

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FGF17 Antibody (500-P152-1MG) in ELISA

Sandwich ELISA: To detect Human FGF-17 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of FGF17 Polyclonal Antibody (Product # 500-P152-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech FGF17 Polyclonal Antibody, Biotin (Product # 500-P152BT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human FGF-17.

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产品信息

500-P152-1MG

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.2 µg/mL

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

酶联免疫吸附实验 (ELISA)

0.5-2.0 µg/mL

产品规格

种属反应

Human

已发表种属

Bovine

宿主/亚型

Rabbit

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived Recombinant Human FGF-17

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

0.1-1.0 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

no preservative

保存条件

-20°C

运输条件

Ambient

RRID

AB_2929498

产品详细信息

AA Sequence of recombinant protein: MTQGENHPSP NFNQYVRDQG AMTDQLSRRQ IREYQLYSRT SGKHVQVTGR RISATAEDGN KFAKLIVETD TFGSRVRIKG AESEKYICMN KRGKLIGKPS GKSKDCVFTE IVLENNYTAF QNARHEGWFM AFTRQGRPRQ ASRSRQNQRE AHFIKRLYQG QLPFPNHAEK QKQFEFVGSA PTRRTKRTRR PQPLT.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human FGF-17. Anti-Human FGF-17-specific antibody was purified by affinity chromatography employing an immobilized Human FGF-17 matrix.

Sandwich ELISA: To detect Human FGF-17 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human FGF-17 (500-P152Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human FGF-17.

Western Blot: To detect Human FGF-17 by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human FGF-17 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

靶标信息

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes including embryonic development cell growth, morphogenesis, tissue repair, tumor growth and invasion. This gene was shown to be prominently expressed in the cerebellum and cortex. The mouse homolog of this gene was localized to specific sites in the midline structures of the forebrain, the midbrain-hindbrain junction, developing skeleton and developing arteries, which suggests a role in central nervous system, bone and vascular development. This gene was referred to as FGF-13 in reference 2, however, its amino acid sequence and chromosomal localization are identical to FGF17.

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