Invitrogen

FGF17 Polyclonal Antibody

高级验证

This Antibody was verified by Neutralization to ensure that the antibody binds to the antigen stated.

查看其他11个FGF17抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

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FGF17 Antibody (PA5-46934) in Neu

Neutralization of FGF17 in NR6R-3T3 mouse fibroblast cell line. Samples were incubated in FGF17 polyclonal antibody (Product # PA5-46934). Recombinant Human FGF-17 stimulates proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human FGF-17 (100 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human FGF-17 Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 6-24 µg/mL in the presence of heparin (1 µg/mL).

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FGF17 Antibody — Neutralization antibody testing demonstrates the specificity of an antibody through a correlation between antibody binding and the activity of the target. Neutralization of human FGF-17 is shown by decrease in CPM (measure of proliferation) with increasing concentrations of FGF-17 polyclonal antibody (PA5-46934). {Neu}

产品信息

PA5-46934

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1 µg/mL

中和实验 (Neu)

6-24 µg/mL

产品规格

种属反应

Human

宿主/亚型

Goat / IgG

分类

Polyclonal

类型

Antibody

抗原

E. coli-derived recombinant human FGF-17 Thr23-Thr216

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

0.2 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 5% trehalose

内含物

No Preservative

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2610199

产品详细信息

In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human (rh) FGF-8b and recombinant mouse FGF-8c is observed and less than 2% cross-reactivity with rhFGF acidic, rhFGF basic, rhFGF-4, rhFGF-5, rhFGF-6, rhFGF-7, rhFGF-10, and rhFGF-18 is observed.

Reconstitute at 0.2 mg/mL in sterile PBS.

Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.

靶标信息

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes including embryonic development cell growth, morphogenesis, tissue repair, tumor growth and invasion. This gene was shown to be prominently expressed in the cerebellum and cortex. The mouse homolog of this gene was localized to specific sites in the midline structures of the forebrain, the midbrain-hindbrain junction, developing skeleton and developing arteries, which suggests a role in central nervous system, bone and vascular development. This gene was referred to as FGF-13 in reference 2, however, its amino acid sequence and chromosomal localization are identical to FGF17.

仅用于科研。不用于诊断过程。未经明确授权不得转售。