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FITC Antibody (MA5-14696) in WB
Western blot analysis of FITC was performed by loading various amounts of a FITC-BSA conjugate and 10 µL PageRuler Plus Prestained Protein Ladder (Product # 26619) per well onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a PVDF membrane using the G2 Fast Blotter (Product # 62288) and blocked with 5% milk in TBST for at least 1 hour at room temperature. FITC-BSA was detected at ~72 kD using a FITC monoclonal antibody (Product # MA5-14696) at a dilution of 1 µg/mL in 5% milk in TBST overnight at 4C on a rocking pla... View More
产品信息
MA5-14696
应用
建议稀释比
已发表文章
产品规格
种属反应
Chemical
宿主/亚型
Mouse
/ IgG2a, lambda
分类
Monoclonal
类型
Antibody
克隆号
#9
抗原
FITC-BSA
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.4, with 1mg/mL BSA
内含物
0.05% sodium azide
保存条件
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_11001761
产品详细信息
Product MA514696 is a smaller package size of MIF2902 (formerly sold as a Seradyn product).
靶标信息
FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins. Anti-FITC antibodies can be used for isolating FITC labeled cells by magnetic microbeads based sorting, as well as removal of background signal.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Fluorescein
