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Invitrogen

FMRP Polyclonal Antibody

3个已发表图片

5 篇参考文献

查看其他21个FMRP抗体

货号 PA5-34584

CNY 4,909.00

100 µg

批量或定制请求

Application

FMRP Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 7

FMRP Antibody (PA5-34584) in ICC/IF

Immunofluorescence analysis of FMRP was performed using Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with FMRP Rabbit Polyclonal Antibody (Product # PA5-34584) at 5 µg/mL dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32731) at a dilution of 1:200... View More

FMRP Antibody in Immunocytochemistry (ICC/IF)

FMRP Antibody in Western Blot (WB)

FMRP Antibody in Western Blot (WB)

FMRP Antibody in Western Blot, Immunohistochemistry (WB, IHC)

FMRP Antibody in Western Blot, Immunoprecipitation (WB, IP)

FMRP Antibody in Western Blot (WB)

Product Image Gallery

FMRP Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of FMRP was performed using Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with FMRP Rabbit Polyclonal Antibody (Product # PA5-34584) at 5 µg/mL dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32731) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the composite image showing nuclear and cytoplasmic localization of FMRP. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

FMRP Antibody in Western Blot (WB) — Western blot was performed using Anti-FMRP Rabbit Polyclonal Antibody (Product # PA5-34584) and a 71 kDa band corresponding to FMRP was observed across cell lines and tissues tested. Whole cell extracts (30 µg lysate) of HEK-293 (Lane 1), U-87 MG (Lane 2), HeLa (Lane 3), A549 (Lane 4), SH-SY5Y (Lane 5), Hep G2 (Lane 6), Mouse Brain (Lane 7) and Rat Brain (Lane 8) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.75 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

FMRP Antibody in Western Blot (WB) — Knockdown of FMRP was achieved by transfecting HEK-293 cells with FMRP specific siRNAs (Silencer® select Product # s5316, s5317). Western blot analysis (Fig. a) was performed whole cell extracts from the HEK-293 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with FMRP Polyclonal Antibody (Product # PA5-34584, 0.1 µg/mL) and Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to FMRP.

FMRP Antibody in Western Blot, Immunohistochemistry (WB, IHC)

FMRP Antibody in Western Blot, Immunoprecipitation (WB, IP) — Fig. 3 FMRP interacts with dynein and is transported in axons together with lysosomes. A , representative images of PLA between FMRP and flag-tagged proteins in DRG neurons transduced with MnT-control or MnT-Dynlrb1. Non-transduced cells were also added as a control. Neurons are labeled with betaIII-tubulin ( green ). The PLA signal is in red . Scale bar represents 10 mum. B , quantification of the PLA experiment in ( A ). PLA signal in the cell bodies and axons was quantified separately. Mean +- SEM, * p < 0.05, ** p < 0.01, *** p < 0.001, ns, not significant, n = 3, one-way ANOVA followed by Tukey's HSD post hoc correction for multiple comparisons. C , Western blot analysis of FMRP immunoprecipitation from axoplasm extracted from mouse sciatic nerve. Coimmunoprecipitation of dynein intermediate chain is revealed by immunostaining with IC74.1 antibody. D , quantification of the pulldown in ( C ). Mean +- SEM, * p < 0.05, n = 3, unpaired t test. E , representative kymograph of DRG neurons transfected with EGFP-FMRP construct (in green ). LysoTracker was added to track lysosomal transport (in red ). The merge between the two channels is in yellow . Scale bar represents 5 mum. F , percentage of anterograde and retrograde versus stationary carriers in the experiment described in ( E ). G , anterograde speed distributions from the experiment described in ( E ). Mean +- SEM, n >14 movies per group over three independent biological repeats

FMRP Antibody in Western Blot (WB) — Genetic depletion of Dynlrb1 impairs FMRP degradation. A , representative images of cell bodies of cultured DRG neurons transduced with shControl or shDynlrb1 constructs. Transduced neurons are labeled by EYFP expressed by the viral constructs (in green ). FMRP granules are visualized by staining with an anti-FMRP antibody ( grayscale) . Scale bars represent 5 mum and 1 mum, respectively. B , quantification of FMRP intensity ( B i) and the number of FMRP-positive granules ( B ii) in the experiment described in ( A ). Mean +- SEM, * p < 0.05, ** p < 0.01, n = 3, unpaired t test. C , Western blot analysis of FMRP protein levels in shControl or shDynlrb1 DRG neurons after incubation with proteasomal inhibitor (MG132), lysosomal inhibitors (leupeptin, pepstatin A, and E-64d) or vehicles for 6 h. beta-actin immunostaining was used to normalize FMRP levels. Viral transduction efficiency was visualized by an anti-GFP antibody. D , quantification of the experiment described in ( C ). Mean +- SEM, n = 5, * p < 0.05, ** p < 0.01, *** p < 0.001, ns not significant, one-way ANOVA followed by Tukey's HSD post hoc correction for multiple comparisons. DRG, dorsal root ganglia; EYFP, enhanced YFP; FMRP, fragile X messenger ribonucleoprotein 1; HSD, honestly significant difference test.

FMRP Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HEK-293 cells were transfected with FMRP siRNA and reduction of signal was observed in Western Blot using FMRP Polyclonal Antibody (Product # PA5-34584). {KD}

产品信息

PA5-34584

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1 µg/mL

查看2篇已发表文章 2篇已发表文章

免疫组化 (IHC)

-

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

5 µg/mL

-

免疫组化（冰冻） (IHC (F))

-

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

5 µg/mL

-

免疫沉淀 (IP)

Assay-Dependent

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Chicken, Gerbil, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide conjugated to KLH derived from within residues 550 to the C-terminus of Human FMRP.

查看免疫原

偶联物

Unconjugated

形式

Liquid

浓度

0.9 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.4, with 1% BSA

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2551936

产品详细信息

For Western Blot, this antibody has non-specific bands at 13 kDa,32 kDa and 68 kDa.

Heat mediated antigen retrieval recommended prior to tissue staining.

This antibody is predicted to react with orangutan based on sequence homology.

靶标信息

FMR1 binds RNA and is associated with polysomes. The protein may be involved in mRNA trafficking from the nucleus to the cytoplasm. A trinucleotide repeat in the 5' UTR is normally found at 6-53 copies, but an expansion to 55-230 repeats is the cause of fragile X syndrome. Expansion of the trinucleotide repeat may also cause one form of premature ovarian failure. Multiple alternatively spliced transcript variants that encode different protein isoforms and which are located in different cellular locations have been described for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Cellular distribution of the Fragile X mental retardation protein in the inner ear: a developmental and comparative study in the mouse, rat, gerbil, and chicken.

The Journal of comparative neurology

Wang X,Fan Q,Yu X,Wang Y

Published figure using FMRP polyclonal antibody (Product # PA5-34584) in Immunohistochemistry

Sun Jan 01 00:00:00 UTC 2023

Cellular distribution of the Fragile X mental retardation protein in the inner ear: a developmental and comparative study in the mouse, rat, gerbil, and chicken.

The Journal of comparative neurology

Wang X,Fan Q,Yu X,Wang Y

Published figure using FMRP polyclonal antibody (Product # PA5-34584) in Immunohistochemistry

Sun Jan 01 00:00:00 UTC 2023

Cellular distribution of the Fragile X mental retardation protein in the inner ear: a developmental and comparative study in the mouse, rat, gerbil, and chicken.

The Journal of comparative neurology

Wang X,Fan Q,Yu X,Wang Y

Published figure using FMRP polyclonal antibody (Product # PA5-34584) in Immunohistochemistry

Sun Jan 01 00:00:00 UTC 2023

Cellular distribution of the Fragile X mental retardation protein in the inner ear: a developmental and comparative study in the mouse, rat, gerbil, and chicken.

The Journal of comparative neurology

Wang X,Fan Q,Yu X,Wang Y

Published figure using FMRP polyclonal antibody (Product # PA5-34584) in Immunohistochemistry

Sun Jan 01 00:00:00 UTC 2023

Cellular distribution of the Fragile X mental retardation protein in the inner ear: a developmental and comparative study in the mouse, rat, gerbil, and chicken.

The Journal of comparative neurology

Wang X,Fan Q,Yu X,Wang Y

Published figure using FMRP polyclonal antibody (Product # PA5-34584) in Immunohistochemistry

Sun Jan 01 00:00:00 UTC 2023

Cellular distribution of the Fragile X mental retardation protein in the inner ear: a developmental and comparative study in the mouse, rat, gerbil, and chicken.

The Journal of comparative neurology

Wang X,Fan Q,Yu X,Wang Y

Published figure using FMRP polyclonal antibody (Product # PA5-34584) in Immunohistochemistry

Sun Jan 01 00:00:00 UTC 2023

Cellular distribution of the Fragile X mental retardation protein in the inner ear: a developmental and comparative study in the mouse, rat, gerbil, and chicken.

The Journal of comparative neurology

Wang X,Fan Q,Yu X,Wang Y

Published figure using FMRP polyclonal antibody (Product # PA5-34584) in Immunohistochemistry

Sun Jan 01 00:00:00 UTC 2023

Cellular distribution of the Fragile X mental retardation protein in the inner ear: a developmental and comparative study in the mouse, rat, gerbil, and chicken.

The Journal of comparative neurology

Wang X,Fan Q,Yu X,Wang Y

Published figure using FMRP polyclonal antibody (Product # PA5-34584) in Immunohistochemistry

Sun Jan 01 00:00:00 UTC 2023

Cellular distribution of the Fragile X mental retardation protein in the inner ear: a developmental and comparative study in the mouse, rat, gerbil, and chicken.

The Journal of comparative neurology

Wang X,Fan Q,Yu X,Wang Y

Published figure using FMRP polyclonal antibody (Product # PA5-34584) in Immunohistochemistry

Sun Jan 01 00:00:00 UTC 2023

FMRP Long-Range Transport and Degradation Are Mediated by Dynlrb1 in Sensory Neurons.

Molecular & cellular proteomics : MCP

El-Agamy SE,Guillaud L,Kono K,Wu Y,Terenzio M

Published figure using FMRP polyclonal antibody (Product # PA5-34584) in Western Blot

Wed Nov 01 00:00:00 UTC 2023

FMRP Long-Range Transport and Degradation Are Mediated by Dynlrb1 in Sensory Neurons.

Molecular & cellular proteomics : MCP

El-Agamy SE,Guillaud L,Kono K,Wu Y,Terenzio M

Published figure using FMRP polyclonal antibody (Product # PA5-34584) in Western Blot

Wed Nov 01 00:00:00 UTC 2023

生物信息学

蛋白别名: FMRP; fragile X mental retardation protein 1; fragile X mental retardation protein 1 homolog; fragile X mental retardation syndrome 1 homolog; fragile X mental retardation-1 protein; Fragile X messenger ribonucleoprotein; Fragile X messenger ribonucleoprotein 1; MGC87458; Protein FMR-1; ragile X mental retardation protein

基因别名: Fmr-1; FMR1; FMRP; FRAXA; POF; POF1

UniProt ID: (Human) Q06787, (Rat) Q80WE1

Entrez Gene ID: (Human) 2332, (Rat) 24948, (Mouse) 14265

G-quadruplex RNA binding chromatin binding RNA binding mRNA binding mRNA 3'-UTR binding protein binding microtubule binding poly(U) RNA binding translation repressor activity translation initiation factor binding RNA strand annealing activity poly(G) binding methylated histone binding siRNA binding miRNA binding RNA stem-loop binding identical protein binding protein homodimerization activity ribosome binding ion channel binding poly(A) RNA binding dynein binding protein heterodimerization activity mRNA 5'-UTR binding sequence-specific mRNA binding nucleic acid binding translation factor

参与通路

regulation of alternative mRNA splicing, via spliceosome positive regulation of receptor internalization mRNA processing cellular response to DNA damage stimulus glutamate receptor signaling pathway RNA splicing negative regulation of translation gene silencing by RNA positive regulation of histone phosphorylation cellular response to UV regulation of mRNA stability modulation by host of viral RNA genome replication positive regulation of translation negative regulation of translational initiation regulation of neurotransmitter secretion mRNA transport regulation of filopodium assembly positive regulation of filopodium assembly regulation of gene silencing by miRNA regulation of dendritic spine development positive regulation of dendritic spine development cellular response to hydroxyurea cellular response to virus regulation of neuronal action potential negative regulation of long term synaptic depression positive regulation of intracellular transport of viral material negative regulation of voltage-gated calcium channel activity positive regulation of proteasomal protein catabolic process positive regulation of mRNA binding negative regulation of synaptic vesicle exocytosis positive regulation of gene silencing by miRNA negative regulation of cytoplasmic translation positive regulation of response to DNA damage stimulus regulation of translation central nervous system development transport dendritic spine development

1x1 image pixel for data collection