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产品信息
MA5-14846
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Non-human primate,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Monoclonal
类型
Antibody
克隆号
S.502.4
抗原
GST-fusion protein corresponding to carboxy-terminal residues of human FoxO1
偶联物
形式
Liquid
浓度
88 µg/mL
纯化类型
Affinity chromatography
保存液
0.01M HEPES, pH 7.5, with 0.15M NaCl, 50% glycerol, 100µg/mL BSA
内含物
<0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_10987316
产品详细信息
Antibodies to this protein (and modification) were previously sold as part of a Thermo Scientific Cellomics High Content Screening Kit. This replacement antibody is now recommended for researchers who need an antibody for high content cell based assays. It has been thoroughly tested and validated for cellular immunofluorescence (IF) applications. Further optimization including the selection of the most appropriate fluorescent Dylight conjugated secondary antibody may have to be performed for your high content assay.
It is not recommended to aliquot this antibody.
靶标信息
FOXO1 (FKHR, ForKHead Box 01) is a member in a subfamily of the forkhead homeotic gene family of transcription factors. Recent experiments have shown that FOXO1 can act as either a coactivator or a corepressor of nuclear receptor activity that is mediated through the LXXLL motif found in the carboxyl terminal region of the FKHR protein. Association of FOXO1 with PAX3 has been implicated in alveolar rhabdomyosarcoma. Recent studies link the anti-tumor activity of FOXO1, the process of autophagy and myogenic growth and differentiation. FOXO1 is the main target of insulin signaling that regulated the metabolic homeostasis in response to oxidative stress. FOXO1 binds to the insulin response element (IRE) with consensus sequence 5'-TT[G/A]TTTTG-3' and the related Daf-16 family binding element (DBE) with consensus sequence 5'- TT[G/A]TTTAC-3'. FOXO1 is a regulator of redox balance, osteoblast numbers and controls bone mass. Further, FOXO1 orchestrates the endocrine function of the skeleton in regulating glucose metabolism, and suppresses the transcriptional activity of RUNX2, an upstream activator of osteocalcin/BGLAP. In hepatocytes, FOXO1 promotes gluconeogenesis by acting together with PPARGC1A to activate the expression of genes such as IGFBP1, G6PC and PPCK1. FOXO1 is an important regulator of cell death acting downstream of CDK1, PKB/AKT1 and SKT4/MST1.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: forkhead box O1a; forkhead box O1A (rhabdomyosarcoma); Forkhead box protein O1; Forkhead box protein O1A; Forkhead in rhabdomyosarcoma; forkhead protein 1; forkhead, Drosophila, homolog of, in rhabdomyosarcoma; FOX01A
基因别名: Afxh; AI876417; FKH1; FKHR; Fkhr1; FOXO1; FOXO1A
UniProt ID: (Human) Q12778, (Rat) G3V7R4, (Mouse) Q9R1E0
Entrez Gene ID: (Human) 2308, (Rat) 84482, (Mouse) 56458
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcription factor activity, transcription factor binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcription coactivator binding
chromatin binding
protein binding
beta-catenin binding
ubiquitin protein ligase binding
sequence-specific DNA binding
protein phosphatase 2A binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
transcription factor binding
negative regulation of transcription from RNA polymerase II promoter
blood vessel development
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
autophagy
apoptotic process
cellular response to DNA damage stimulus
insulin receptor signaling pathway
cellular response to starvation
positive regulation of autophagy
cellular response to insulin stimulus
negative regulation of stress-activated MAPK cascade
cellular response to oxidative stress
regulation of gluconeogenesis by regulation of transcription from RNA polymerase II promoter
regulation of cell proliferation
glucose homeostasis
positive regulation of apoptotic process
negative regulation of apoptotic process
negative regulation of fat cell differentiation
positive regulation of gluconeogenesis
positive regulation of protein catabolic process
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
cellular response to hyperoxia
cellular response to nitric oxide
negative regulation of canonical Wnt signaling pathway
neuronal stem cell population maintenance
regulation of nucleic acid-templated transcription
regulation of neural precursor cell proliferation
regulation of reactive oxygen species metabolic process
transcription, DNA-templated
anatomical structure morphogenesis
cell differentiation
temperature homeostasis
cellular glucose homeostasis
protein acetylation
endocrine pancreas development
fat cell differentiation
negative regulation of transcription, DNA-templated
enamel mineralization
cellular response to hydrogen peroxide
cellular response to cold
cellular response to dexamethasone stimulus
response to fluoride
regulation of energy homeostasis
