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FPR1 (extracellular) Antibody (AFR-001-200UL) in WB
Western blot analysis of human HL-60 promyelocytic leukemia (lanes 1 and 5), human THP-1 acute monocytic leukemia (lanes 2 and 6), human T-84 colorectal carcinoma (lanes 3 and 7) and human U-87 MG glioblastoma (lanes 4 and 8) cell lysates: - 1-4. Anti-Human FPR1 (extracellular) Antibody (#AFR-001), (1:200).5-8. Anti-Human FPR1 (extracellular) Antibody , preincubated with Human FPR1 (extracellular) Blocking ... View More
产品信息
AFR-001-200UL
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
(C)NFSPWTNDPKERIN, corresponding to amino acid residues 179-192 of human FPR1, 2nd extracellular loop
偶联物
Unconjugated
形式
Lyophilized
浓度
0.8 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4, with 1% BSA
内含物
0.05% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
靶标信息
FPR1 is a member of chemoattractant/chemokine GPCRs expressed mainly on leukocytes, neutrophils and monocytes. FPR1 is a seven transmembrane pertussis toxin-sensitive GPCR and binds to formylated peptides generated by bacteria resulting in leukocyte trafficking to the site of inflammation. Ligand binding to FPR results in activation of multiple effectors including phospholipase C leading to calcium mobilization and PKC activation. Other implications of FPR signaling include polarization of actin cytoskeleton, activation of various integrins, increased cell migration, phagocytosis, degranulation and release of reactive oxygen intermediates.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: fMet-Leu-Phe receptor; fMLP receptor; FPR; Fpr receptor; FPR-1; N-formyl peptide receptor; N-formylpeptide chemoattractant receptor
基因别名: FMLP; FPR; FPR1
UniProt ID: (Human) P21462
Entrez Gene ID: (Human) 2357
activation of MAPK activity
movement of cell or subcellular component
chemotaxis
signal transduction
G-protein coupled receptor signaling pathway
adenylate cyclase-modulating G-protein coupled receptor signaling pathway
phospholipase C-activating G-protein coupled receptor signaling pathway
nitric oxide mediated signal transduction
