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Invitrogen
FTO Recombinant Rabbit Monoclonal Antibody (JM98-30)
高级验证
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
图: 1 / 8
FTO Antibody (MA5-32785) in ICC/IF
Immunofluorescence analysis of Alpha-ketoglutarate-dependent dioxygenase FTO was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with FTO Recombinant Rabbit Monoclonal Antibody (JM98-30) (Product # MA5-32785) at 1:200 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adso... View More
产品信息
MA5-32785
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
JM98-30
抗原
Synthetic peptide within Human FTO aa 1-50
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2810061
产品详细信息
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
靶标信息
FTO is a novel protein and a member of the non-heme dioxygenase (Fe(II)-and 2-oxoglutarate-dependent dioxygenases) superfamily. Amino acid conservation patterns support this hypothesis and indicates that both 2-oxoglutarate and iron should be important for FTO function. Though not much is yet known about FTO, it is thought that the protein may somehow modify the activity of genes involved in metabolism and fat storage, which in turn may influence a person's risk of obesity.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: AlkB homolog 9; Alpha-ketoglutarate-dependent dioxygenase FTO; Fat mass and obesity-associated protein; fatso; m6A(m)-demethylase FTO; MGC5149; mRNA (2'-O-methyladenosine-N(6)-)-demethylase FTO; mRNA N(6)-methyladenosine demethylase FTO; tRNA N1-methyl adenine demethylase FTO; U6 small nuclear RNA (2'-O-methyladenosine-N(6)-)-demethylase FTO; U6 small nuclear RNA N(6)-methyladenosine-demethylase FTO
基因别名: ALKBH9; BMIQ14; FTO; GDFD; KIAA1752
UniProt ID: (Human) Q9C0B1
Entrez Gene ID: (Human) 79068
temperature homeostasis
DNA dealkylation involved in DNA repair
regulation of lipid storage
oxidative single-stranded DNA demethylation
oxidative single-stranded RNA demethylation
regulation of multicellular organism growth
RNA repair
regulation of respiratory system process
adipose tissue development
regulation of white fat cell proliferation
oxidative demethylation
DNA demethylation
regulation of brown fat cell differentiation
