Invitrogen

Factor X Recombinant Rabbit Monoclonal Antibody (3F2)

Factor X Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Factor X Antibody (MA5-49874) in IHC (P)

Immunohistochemistry analysis with a Factor X monoclonal antibody (Product # MA5-49874) using a dilution of 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 min at RT. Then primary antibody (1% BSA) was inc... View More

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产品信息

MA5-49874

应用

建议稀释比

已发表文章

免疫组化（石蜡） (IHC (P))

1:50-1:200

酶联免疫吸附实验 (ELISA)

Assay-dependent

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

3F2

抗原

A synthesized peptide derived from human Factor X

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.9 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C or -80°C if preferred

运输条件

Wet ice

RRID

AB_3092768

靶标信息

F10 undergoes multiple processing steps before its preproprotein is converted to a mature two-chain form by the excision of the tripeptide RKR. Two chains of the factor are held together by 1 or more disulfide bonds; the light chain contains 2 EGF-like domains, while the heavy chain contains the catalytic domain which is structurally homologous to those of the other hemostatic serine proteases. The mature factor is activated by the cleavage of the activation peptide by factor IXa (in the intrisic pathway), or by factor VIIa (in the extrinsic pathway). The activated factor then converts prothrombin to thrombin in the presence of factor Va, Ca+2, and phospholipid during blood clotting. Mutations of this gene result in factor X deficiency, a hemorrhagic condition of variable severity.

仅用于科研。不用于诊断过程。未经明确授权不得转售。