Rockland

Fetuin Polyclonal Antibody

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Fetuin Antibody (200-101-456) in WB

Western blot of Fetuin. Anti-Human Fetuin antibody, generated by immunization with mature protein, was tested by western blot against fetuin in purified preparations and in human plasma. Lane 1 contains 250 ng of purified human fetuin. Lane 2 contains 5 µl of a 1:50 dilution of human serum. Dilution of Anti-Human Fetuin antibody between 1:10,000 and 1:20,000 showed strong reactivity by western blot. In this blot the antibody was used at a 1:10,000 dilution incubated 1 h at room temperature in 1% BSA in TTBS. Detection occurred using a 1:5... View More

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产品信息

200-101-456

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:10,000-1:20,000

酶联免疫吸附实验 (ELISA)

1:40,000-1:200,000

免疫沉淀 (IP)

1:10,000-1:20,000

产品规格

种属反应

Human

宿主/亚型

Goat / IgG

分类

Polyclonal

类型

Antibody

抗原

This purified antibody was prepared from rabbit serum after repeated immunizations with a recombinant human fetuin (a₂-HS glycoprotein) processed to remove a 40 amino acid residue bridging peptide resulting in the mature form of the protein.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

1 mg/mL

纯化类型

Ion-exchange chromatography

保存液

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

内含物

0.01% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum.

靶标信息

Alpha2-HS glycoprotein (AHSG), a glycoprotein present in the serum, is synthesized by hepatocytes. The AHSG molecule consists of two polypeptide chains, which are both cleaved from a proprotein encoded from a single mRNA. It is involved in several functions, such as endocytosis, brain development and the formation of bone tissue. The protein is commonly present in the cortical plate of the immature cerebral cortex and bone marrow hemopoietic matrix, and it has therefore been postulated that it participates in the development of the tissues. However, its exact significance is still obscure.

仅用于科研。不用于诊断过程。未经明确授权不得转售。