Invitrogen

Fibrillarin Monoclonal Antibody (38F3)

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

4个已发表图片

9 篇参考文献

查看其他23个Fibrillarin抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

Fibrillarin Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 14

Fibrillarin Antibody (MA3-16771) in ICC/IF

Immunofluorescence analysis of Fibrillarin was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Fibrillarin Monoclonal Antibody (38F3) (Product # MA3-16771) at 1:250 dilution in 0.1% BSA, incubated at 4 degr... View More

Fibrillarin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Fibrillarin Antibody in Western Blot (WB)

Fibrillarin Antibody in Flow Cytometry (Flow)

Product Image Gallery

产品信息

MA3-16771

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:5,000

查看5篇已发表文章 5篇已发表文章

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

1:500-1:1,000

免疫组化（冰冻） (IHC (F))

1:500-1:1,000

免疫细胞化学 (ICC/IF)

1:500-1:1,000

查看3篇已发表文章 3篇已发表文章

流式细胞分析 (Flow)

1:20-1:400

产品规格

种属反应

Bovine, C. elegans, Chicken, Fruit fly, Horse, Human, Mouse, Plant, Pig, Rat, Yeast

已发表种属

Human, Mouse, Plant, Rat

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

38F3

抗原

Yeast nuclear preparation (S. cerevisiae)

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

Conc. not determined

保存液

tissue culture supernatant

内含物

0.035% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2105791

产品详细信息

Suggested positive control: antigen standard for FBL (transient overexpression lysate).

靶标信息

Nop1p / Fibrillarin was originally identified as a nucleolar protein of bakers yeast, Saccharomyces cerevisiae (accession P15646). The Nop1p protein is essential for yeast viability and is localized in the nucleoli. The human homologue of Nop1p is fibrillarin (accession P22087) a component of the nucleolar small nuclear ribonucleoprotein (snRNP) particle. The human fibrillarin gene is located on chromosome 19 (19q13. 1).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

RNA-interference screen for p53 regulators unveils a role of WDR75 in ribosome biogenesis.

Cell death and differentiation

Moudry P,Chroma K,Bursac S,Volarevic S,Bartek J

MA3-16771 was used in Immunocytochemistry-immunofluorescence to reveal a crucial positive role of WDR75 in ribosome biogenesis and provide a resource of human ribosomal factors the malfunction of which affects p53.

Tue Mar 01 00:00:00 EST 2022

MALAT1 as a Regulator of the Androgen-Dependent Choline Kinase A Gene in the Metabolic Rewiring of Prostate Cancer.

Cancers

De Martino S,Iorio E,Cencioni C,Aiello A,Spallotta F,Chirico M,Pisanu ME,Grassi C,Pontecorvi A,Gaetano C,Nanni S,Farsetti A

MA3-16771 was used in Western Blotting to support the role of MALAT1 as a CHKA activator through putative association with the liganded or unliganded AR, unveiling its targeting as a therapeutic option from a metabolic rewiring perspective.

Sun Jun 12 00:00:00 EDT 2022

Complete loss of H3K9 methylation dissolves mouse heterochromatin organization.

Nature communications

Montavon T,Shukeir N,Erikson G,Engist B,Onishi-Seebacher M,Ryan D,Musa Y,Mittler G,Meyer AG,Genoud C,Jenuwein T

MA3-16771 was used in Immunocytochemistry to reveal a definitive function for H3K9 methylation in protecting heterochromatin organization and genome integrity.

Fri Jul 16 00:00:00 EDT 2021

Complete loss of H3K9 methylation dissolves mouse heterochromatin organization.

Nature communications

Montavon T,Shukeir N,Erikson G,Engist B,Onishi-Seebacher M,Ryan D,Musa Y,Mittler G,Meyer AG,Genoud C,Jenuwein T

MA3-16771 was used in Immunocytochemistry to reveal a definitive function for H3K9 methylation in protecting heterochromatin organization and genome integrity.

Fri Jul 16 00:00:00 EDT 2021

Proteomic Identification of an Endogenous Synaptic SUMOylome in the Developing Rat Brain.

Frontiers in molecular neuroscience

Pronot M,Kieffer F,Gay AS,Debayle D,Forquet R,Poupon G,Schorova L,Martin S,Gwizdek C

MA3-16771 was used in Western Blotting to identify and characterize 803 candidate SUMO2/3 target proteins in the synaptic proteome of the mammalian brain, revealing SUMO2/3 as a central regulator of synaptic organization and function during brain development.

Sat Dec 11 00:00:00 EST 2021

H19-Dependent Transcriptional Regulation of β3 and β4 Integrins Upon Estrogen and Hypoxia Favors Metastatic Potential in Prostate Cancer.

International journal of molecular sciences

Bacci L,Aiello A,Ripoli C,Loria R,Pugliese D,Pierconti F,Rotili D,Strigari L,Pinto F,Bassi PF,Mai A,Grassi C,Pontecorvi A,Falcioni R,Farsetti A,Nanni S

MA3-16771 was used in Western Blot to dissect the contribution of long non-coding RNA H19 to PCa metastatic potential under combined stimuli.

Sat Aug 17 00:00:00 EDT 2019

Nucleoporin 153 regulates estrogen-dependent nuclear translocation of endothelial nitric oxide synthase and estrogen receptor beta in prostate cancer.

Oncotarget

Re A,Colussi C,Nanni S,Aiello A,Bacci L,Grassi C,Pontecorvi A,Farsetti A

Published figure using Fibrillarin monoclonal antibody (Product # MA3-16771) in Western Blot

Fri Jun 15 00:00:00 EDT 2018

Optimized protocol for combined PALM-dSTORM imaging.

Scientific reports

Glushonkov O,Réal E,Boutant E,Mély Y,Didier P

Published figure using Fibrillarin monoclonal antibody (Product # MA3-16771) in Immunofluorescence

Fri Jun 08 00:00:00 EDT 2018

Olfactory memory is enhanced in mice exposed to extremely low-frequency electromagnetic fields via Wnt/β-catenin dependent modulation of subventricular zone neurogenesis.

Scientific reports

Mastrodonato A,Barbati SA,Leone L,Colussi C,Gironi K,Rinaudo M,Piacentini R,Denny CA,Grassi C

MA3-16771 was used in Western Blotting to assay whether electromagnetic fields stimulation affects olfactory memory by modulating neurogenesis in the subventricular zone of the lateral ventricle, and investigated the underlying molecular mechanisms.

Wed Jan 10 00:00:00 EST 2018

40S ribosome biogenesis co-factors are essential for gametophyte and embryo development.

PloS one

Missbach S,Weis BL,Martin R,Simm S,Bohnsack MT,Schleiff E

MA3-16771 was used in immunohistochemistry to study the roles of 5 potential co-factors of Arabidopsis ribosome biogenesis

Wed Sep 04 00:00:00 EDT 2013

生物信息学

蛋白别名: 34 kDa nucleolar scleroderma antigen; 34-kD nucleolar scleroderma antigen; AT20009p; CG5725-PA; CG5725-PB; CG5725-PC; CG5725-PD; CG5725-PE; CG5725-PF; CG5725-PG; fbl-PA; fbl-PB; fbl-PC; fbl-PD; fbl-PE; fbl-PF; fbl-PG; Histone-glutamine methyltransferase; LD24618p; LP11571p; Nucleolar protein 1; pantothenate kinase; RNA, U3 small nucleolar interacting protein 1; rRNA 2'-O-methyltransferase fibrillarin; U6 snRNA 2'-O-methyltransferase fibrillarin

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基因别名: AL022665; CELE_T01C3.7; CG5725; Dmel\CG5725; Dmel_CG5725; dPANK; dPANK/Fbl; FBL; FIB; fib-1; FIB1; FLRN; PANK; RNU3IP1; T01C3.7

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功能

参与通路

box C/D snoRNA 3'-end processing osteoblast differentiation rRNA processing tRNA processing rRNA methylation snoRNA localization histone glutamine methylation RNA processing snoRNA metabolic process methylation mitotic cell cycle response to oxidative stress chromosome segregation spermatogenesis imaginal disc-derived wing morphogenesis coenzyme A biosynthetic process regulation of cytokinesis locomotion locomotor rhythm oogenesis meiotic cell cycle membrane organization triglyceride homeostasis cellular response to gamma radiation nucleologenesis positive regulation of translation

Fibrillarin Monoclonal Antibody (38F3)

Fibrillarin Antibody (MA3-16771) in ICC/IF

产品信息

MA3-16771

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（石蜡） (IHC (P))

免疫组化（冰冻） (IHC (F))

免疫细胞化学 (ICC/IF)

流式细胞分析 (Flow)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

抗原

偶联物

形式

浓度

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

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