Invitrogen

Fibrillin 1 Polyclonal Antibody

Fibrillin 1 Antibody in Immunocytochemistry (ICC/IF)

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Fibrillin 1 Antibody (PA5-99225) in ICC/IF

Immunofluorescent analysis of Fibrillin 1 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and Fibrillin 1 polyclonal antibody (Product # PA5-99225) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor ... View More

Fibrillin 1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Fibrillin 1 Antibody in Western Blot (WB)

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产品信息

PA5-99225

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

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免疫组化（石蜡） (IHC (P))

1:50-1:200

免疫细胞化学 (ICC/IF)

1:100-1:500

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Not Applicable

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human FBN1(Accession P35555), corresponding to amino acid residues V2823-H2871.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

RRID

AB_2818158

产品详细信息

Antibody detects endogenous levels of total Fibrillin 1.

靶标信息

Fibrillin 1 is an extracellular acidic protein with a high cysteine content and an extended thread-like shape with mosaic composition of different types of extracellular modules. Most of the fibrillin molecule is contributed by 47 epidermal growth factor-like (EGF-like) repeats; 43 of them have a consensus sequence for calcium binding (cb). Other modules including 8-cysteine motifs (seven), hybrid motifs (two), and unique domains (three) are interspersed throughout the molecule[3-7]. Fibrillin 1 contains a proline-rich region close to the N-terminus that is replaced by a glycine-rich region in Fibrillin 2. The fibrillins are found throughout the connective tissue as integral components of extended fibrils.

仅用于科研。不用于诊断过程。未经明确授权不得转售。