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Invitrogen
Fibronectin Polyclonal Antibody
高级验证
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
图: 1 / 5
Fibronectin Antibody (PA1-23693) in ICC/IF
Immunofluorescence analysis of Fibronectin was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Fibronectin Monoclonal Antibody (3F12) (Product # MA5-14737) at 1:100 dilution in 0.1% BSA, incubated at ... View More
产品信息
PA1-23693
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Pig
已发表种属
Mouse,
Pig,
Sheep
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Highly purified, human fibronectin from plasma.
偶联物
形式
Liquid
浓度
2 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.4, with 1% BSA
内含物
0.05% sodium azide
保存条件
4° C, do not freeze
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_558935
产品详细信息
This antibody does not cross-react with Fibrinogen or other serum proteins.
Prolonged fixation in buffered formalin may destroy the epitope. Auto/Zyme predigestion is recommended when using this antibody with formalin-fixed paraffin-embedded tissue sections. Suggested positive controls for this product are human tonsil, liver, skin or kidney.
靶标信息
Fibronectin is a disulfide-bonded dimer with a molecular weight range of 230-250 kDa. In the extracellular matrix of several connective tissues and vessels, fibronectin is present as an insoluble protein that is extensively cross-linked by interchain disulfide bonds forming high molecular mass polymers. Fibronectin is most abundant during embryonic development and tissue remodeling. Fibronectin is also present at high concentrations as a soluble plasma protein. Fibronectin is organized as a linear series of repeating modules which form domains for interaction with fibronectin itself, other matrix components (e.g. collagen and heparin) and receptors on cells (e.g. integrins). Fibronectin is present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. Fibronectin has been implicated in carcinoma development in lung cancer. Further, Fibronectin expression is increased especially in non-small cell lung carcinoma. The adhesion of lung carcinoma cells to Fibronectin enhances tumorgenecity and confers resistance to apoptosis induced by standard chemotherapeutic agents. The gene encoding Fibronectin has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some Fibronectin variants has not been determined.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: CIG; Cold insoluble globulin (CIG); Cold-insoluble globulin; DKFZp686F10164; DKFZp686H0342; DKFZp686I1370; DKFZp686O13149; ferritin L subunit; ferritin L-chain; Fibronectin; FN; Migration stimulating factor (MSF); migration-stimulating factor
基因别名: CIG; ED-B; FINC; FN; FN1; FNZ; GFND; GFND2; LETS; MSF
UniProt ID: (Human) P02751
Entrez Gene ID: (Pig) 397620, (Human) 2335
angiogenesis
cell activation
regulation of protein phosphorylation
platelet degranulation
response to ischemia
acute-phase response
cell-substrate junction assembly
cell adhesion
calcium-independent cell-matrix adhesion
positive regulation of cell proliferation
glial cell migration
regulation of cell shape
response to wounding
response to ozone
positive regulation of gene expression
positive regulation of peptidase activity
peptide cross-linking
extracellular matrix disassembly
extracellular matrix organization
integrin activation
substrate adhesion-dependent cell spreading
cellular response to vascular endothelial growth factor stimulus
endodermal cell differentiation
cellular response to platelet-derived growth factor stimulus
wound healing
negative regulation of apoptotic process
positive regulation of axon extension
positive regulation of fibroblast proliferation
leukocyte migration
positive regulation of chemotaxis
response to glucocorticoid
regulation of ERK1 and ERK2 cascade
cellular response to lipopolysaccharide
cellular response to mercury ion
cellular response to glucose stimulus
cellular response to interleukin-1
cellular response to prostaglandin E stimulus
cellular response to transforming growth factor beta stimulus
cellular response to BMP stimulus
positive regulation of substrate-dependent cell migration, cell attachment to substrate
negative regulation of transforming growth factor-beta secretion
