Invitrogen

Fibronectin Polyclonal Antibody

高级验证

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

9个已发表图片

12 篇参考文献

查看其他54个Fibronectin抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

Fibronectin Antibody in Western Blot (WB)

图: 1 / 21

Fibronectin Antibody (PA5-29578) in WB

Knockout of Fibronectin was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR616644_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Western blot analysis of Fibronectin was performed by loading 30 µg of Hep G2 Wild Type (Lane 1), Hep G2 Wild Type treated with 1X PTI for 4hrs (Lane 2),Hep G2 Cas9 (Lane 3), Hep G2 Cas9 treated with 1X PTI for 4hrs (Lane 4), Hep G2 Fibronectin KO (Lane 5) and Hep G2 Fibronectin KO treated with 1X PTI for 4hrs (Lane 6) whole cell extracts. The s... View More

Fibronectin Antibody in Immunocytochemistry (ICC/IF)

Fibronectin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Fibronectin Antibody in Immunoprecipitation (IP)

Fibronectin Antibody in Immunohistochemistry (IHC)

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产品信息

PA5-29578

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

查看6篇已发表文章 6篇已发表文章

免疫组化 (IHC)

查看3篇已发表文章 3篇已发表文章

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫组化（冰冻） (IHC (F))

Assay-dependent

免疫细胞化学 (ICC/IF)

1:100-1:1,000

查看2篇已发表文章 2篇已发表文章

流式细胞分析 (Flow)

查看1篇已发表文章 1篇已发表文章

酶联免疫吸附实验 (ELISA)

1:1000-1:10000

免疫沉淀 (IP)

1:100-1:500

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 396 and 689 of Human Fibronectin

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.48 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol, 1% BSA

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2547054

产品详细信息

Recommended positive controls: HepG2, HepG2(10 mM Nicotinamide and 0.4 mM Trichostatin A treatment for 48 hr), HeLa mock and shFN1, mouse plasma.

Predicted reactivity: Mouse (92%), Rat (92%), Chicken (88%), Bovine (92%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

Fibronectin is a disulfide-bonded dimer with a molecular weight range of 230-250 kDa. In the extracellular matrix of several connective tissues and vessels, fibronectin is present as an insoluble protein that is extensively cross-linked by interchain disulfide bonds forming high molecular mass polymers. Fibronectin is most abundant during embryonic development and tissue remodeling. Fibronectin is also present at high concentrations as a soluble plasma protein. Fibronectin is organized as a linear series of repeating modules which form domains for interaction with fibronectin itself, other matrix components (e.g. collagen and heparin) and receptors on cells (e.g. integrins). Fibronectin is present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. Fibronectin has been implicated in carcinoma development in lung cancer. Further, Fibronectin expression is increased especially in non-small cell lung carcinoma. The adhesion of lung carcinoma cells to Fibronectin enhances tumorgenecity and confers resistance to apoptosis induced by standard chemotherapeutic agents. The gene encoding Fibronectin has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some Fibronectin variants has not been determined.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Evaluation of Epithelial-Mesenchymal Transition Markers in Autoimmune Thyroid Diseases.

International journal of molecular sciences

Sacristán-Gómez P,Serrano-Somavilla A,Castro-Espadas L,Sánchez de la Blanca Carrero N,Sampedro-Núñez M,Muñoz-De-Nova JL,Molina-Jiménez F,Rosell A,Marazuela M,Martínez-Hernández R

Published figure using Fibronectin polyclonal antibody (Product # PA5-29578) in Western Blot

Wed Feb 08 00:00:00 EST 2023

Evaluation of Epithelial-Mesenchymal Transition Markers in Autoimmune Thyroid Diseases.

International journal of molecular sciences

Sacristán-Gómez P,Serrano-Somavilla A,Castro-Espadas L,Sánchez de la Blanca Carrero N,Sampedro-Núñez M,Muñoz-De-Nova JL,Molina-Jiménez F,Rosell A,Marazuela M,Martínez-Hernández R

Published figure using Fibronectin polyclonal antibody (Product # PA5-29578) in Western Blot

Wed Feb 08 00:00:00 EST 2023

Evaluation of Epithelial-Mesenchymal Transition Markers in Autoimmune Thyroid Diseases.

International journal of molecular sciences

Sacristán-Gómez P,Serrano-Somavilla A,Castro-Espadas L,Sánchez de la Blanca Carrero N,Sampedro-Núñez M,Muñoz-De-Nova JL,Molina-Jiménez F,Rosell A,Marazuela M,Martínez-Hernández R

Published figure using Fibronectin polyclonal antibody (Product # PA5-29578) in Western Blot

Wed Feb 08 00:00:00 EST 2023

Evaluation of Epithelial-Mesenchymal Transition Markers in Autoimmune Thyroid Diseases.

International journal of molecular sciences

Sacristán-Gómez P,Serrano-Somavilla A,Castro-Espadas L,Sánchez de la Blanca Carrero N,Sampedro-Núñez M,Muñoz-De-Nova JL,Molina-Jiménez F,Rosell A,Marazuela M,Martínez-Hernández R

Published figure using Fibronectin polyclonal antibody (Product # PA5-29578) in Western Blot

Wed Feb 08 00:00:00 EST 2023

Paclitaxel Induces Epidermal Molecular Changes and Produces Subclinical Alterations in the Skin of Gynecological Cancer Patients.

Cancers

Montero P,Pérez-Leal M,Pérez-Fidalgo JA,Sanz C,Estornut C,Roger I,Milara J,Cervantes A,Cortijo J

PA5-29578 was used in Western Blot to suggest that paclitaxel modifies gene and protein expression of skin-related molecular markers, and impairs different physical, physiological, and biomechanical properties of the skin of cancer patients at a subclinical level.

Wed Feb 23 00:00:00 EST 2022

Paclitaxel Induces Epidermal Molecular Changes and Produces Subclinical Alterations in the Skin of Gynecological Cancer Patients.

Cancers

Montero P,Pérez-Leal M,Pérez-Fidalgo JA,Sanz C,Estornut C,Roger I,Milara J,Cervantes A,Cortijo J

Wed Feb 23 00:00:00 EST 2022

Development of skeletal muscle atrophy and intermuscular adipose tissue in patients with early breast cancer treated with chemotherapy.

American journal of physiology. Cell physiology

Mallard J,Hucteau E,Bender L,Charlot A,Debrut L,Pflumio C,Trensz P,Schott R,Favret F,Pivot X,Hureau TJ,Pagano AF

PA5-29578 was used in Western Blotting to demonstrate both IMAT development and muscle atrophy in patients with breast cancer who were treated with chemotherapy.

Sat Oct 01 00:00:00 EDT 2022

Human Mesenchymal Stromal Cell-Derived Exosomes Promote In Vitro Wound Healing by Modulating the Biological Properties of Skin Keratinocytes and Fibroblasts and Stimulating Angiogenesis.

International journal of molecular sciences

Tutuianu R,Rosca AM,Iacomi DM,Simionescu M,Titorencu I

Published figure using Fibronectin polyclonal antibody (Product # PA5-29578) in Western Blot

Wed Jun 09 00:00:00 EDT 2021

Human Mesenchymal Stromal Cell-Derived Exosomes Promote In Vitro Wound Healing by Modulating the Biological Properties of Skin Keratinocytes and Fibroblasts and Stimulating Angiogenesis.

International journal of molecular sciences

Tutuianu R,Rosca AM,Iacomi DM,Simionescu M,Titorencu I

Published figure using Fibronectin polyclonal antibody (Product # PA5-29578) in Western Blot

Wed Jun 09 00:00:00 EDT 2021

Human Mesenchymal Stromal Cell-Derived Exosomes Promote In Vitro Wound Healing by Modulating the Biological Properties of Skin Keratinocytes and Fibroblasts and Stimulating Angiogenesis.

International journal of molecular sciences

Tutuianu R,Rosca AM,Iacomi DM,Simionescu M,Titorencu I

Published figure using Fibronectin polyclonal antibody (Product # PA5-29578) in Western Blot

Wed Jun 09 00:00:00 EDT 2021

A step toward engineering thick tissues: Distributing microfibers within 3D printed frames.

Journal of biomedical materials research. Part A

Molde J,Steele JAM,Pastino AK,Mahat A,Murthy NS,Kohn J

Published figure using Fibronectin polyclonal antibody (Product # PA5-29578) in Immunocytochemistry

Sun Mar 01 00:00:00 EST 2020

A step toward engineering thick tissues: Distributing microfibers within 3D printed frames.

Journal of biomedical materials research. Part A

Molde J,Steele JAM,Pastino AK,Mahat A,Murthy NS,Kohn J

Published figure using Fibronectin polyclonal antibody (Product # PA5-29578) in Immunocytochemistry

Sun Mar 01 00:00:00 EST 2020

1,25 Dihydroxyvitamin D3 Enhances the Antifibroid Effects of Ulipristal Acetate in Human Uterine Fibroids.

Reproductive sciences (Thousand Oaks, Calif.)

Ali M,Shahin SM,Sabri NA,Al-Hendy A,Yang Q

PA5-29578 was used in Western Blotting to suggest that combining vitamin D3 with ulipristal acetate produces additional and orchestrated anti-uterine fibroid effects.

Sat Jun 01 00:00:00 EDT 2019

Diet-induced hepatic steatosis abrogates cell-surface LDLR by inducing de novo PCSK9 expression in mice.

The Journal of biological chemistry

Lebeau PF,Byun JH,Platko K,MacDonald ME,Poon SV,Faiyaz M,Seidah NG,Austin RC

PA5-29578 was used in Immunohistochemistry to examine the effect of hepatic steatosis on LDLR expression and circulating LDL cholesterol levels.

Fri Jun 07 00:00:00 EDT 2019

Morphological and Molecular Changes in Juvenile Normal Human Fibroblasts Exposed to Simulated Microgravity.

Scientific reports

Buken C,Sahana J,Corydon TJ,Melnik D,Bauer J,Wehland M,Krüger M,Balk S,Abuagela N,Infanger M,Grimm D

PA5-29578 was used in Western Blotting to show simulated microgravity causes changes in the cytoskeleton, extracellular matrix, focal adhesion and growth behaviour of normal human dermal fibroblasts and to identify the factors involved in the assembly of the fibroblasts.

Thu Aug 15 00:00:00 EDT 2019

Morphological and Molecular Changes in Juvenile Normal Human Fibroblasts Exposed to Simulated Microgravity.

Scientific reports

Buken C,Sahana J,Corydon TJ,Melnik D,Bauer J,Wehland M,Krüger M,Balk S,Abuagela N,Infanger M,Grimm D

Thu Aug 15 00:00:00 EDT 2019

In vitro assessment of a novel, hypothermically stored amniotic membrane for use in a chronic wound environment.

International wound journal

McQuilling JP,Vines JB,Mowry KC

Published figure using Fibronectin polyclonal antibody (Product # PA5-29578) in Immunohistochemistry

Fri Dec 01 00:00:00 EST 2017

生物信息学

蛋白别名: CIG; Cold insoluble globulin (CIG); Cold-insoluble globulin; DKFZp686F10164; DKFZp686H0342; DKFZp686I1370; DKFZp686O13149; ferritin L subunit; ferritin L-chain; Fibronectin; FN; Migration stimulating factor (MSF); migration-stimulating factor

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基因别名: CIG; E330027I09; ED-B; FIBNEC; FINC; FN; Fn-1; FN1; FNZ; GFND; GFND2; LETS; MSF

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UniProt ID: (Human) P02751, (Mouse) P11276

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Entrez Gene ID: (Human) 2335, (Mouse) 14268, (Rat) 25661

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功能