Invitrogen

Follistatin Polyclonal Antibody, Biotin, PeproTech®

Follistatin Antibody in Western Blot (WB)

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Follistatin Antibody (500-P207BT-25UG) in WB

Western Blot: To detect Human Follistatin by Western Blot analysis Follistatin Polyclonal Antibody, Biotin (Product # 500-P207BT-1MG) can be used at a concentration of 0.1-0.2 mg/mL. When used in conjunction with compatible development reagents the detection limit for Recombinant Human Follistatin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

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产品信息

500-P207BT-25UG

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.2 mg/mL

酶联免疫吸附实验 (ELISA)

0.25-1.0 µg/mL

产品规格

种属反应

Human

宿主/亚型

Rabbit

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived Recombinant Human Follistatin

查看免疫原

偶联物

Biotin Biotin Biotin

形式

Lyophilized

浓度

0.1-1.0 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

no preservative

保存条件

-20°C

运输条件

Ambient

RRID

AB_2929814

产品详细信息

AA Sequence of recombinant protein: GNCWLRQAKN GRCQVLYKTE LSKEECCSTG RLSTSWTEED VNDNTLFKWM IFNGGAPNCI PCKETCENVD CGPGKKCRMN KKNKPRCVCA PDCSNITWKG PVCGLDGKTY RNECALLKAR CKEQPELEVQ YQGRCKKTCR DVFCPGSSTC VVDQTNNAYC VTCNRICPEP ASSEQYLCGN DGVTYSSACH LRKATCLLGR SIGLAYEGKC IKAKSCEDIQ CTGGKKCLWD FKVGRGRCSL CDELCPDSKS DEPVCASDNA TYASECAMKE AACSSGVLLE VKHSGSCN.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Follistatin. Anti-Human Follistatin-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA: To detect Human Follistatin by sandwich ELISA (using 100 mL/well) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti- Human Follistatin (500-P207) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human Follistatin.

Western Blot: To detect Human Follistatin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 mg/mL. When used in conjunction with compatible development reagents the detection limit for Recombinant Human Follistatin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

500-P207BT-1MG will be provided as 2 x 500 µg

靶标信息

Follistatin is a single-chain gonadal protein that specifically inhibits follicle-stimulating hormone release. The single FST gene encodes two isoforms, FST317 and FST344 containing 317 and 344 amino acids respectively, resulting from alternative splicing of the precursor mRNA. In a study in which 37 candidate genes were tested for linkage and association with polycystic ovary syndrome (PCOS) or hyperandrogenemia in 150 families, evidence was found for linkage between PCOS and follistatin.

仅用于科研。不用于诊断过程。未经明确授权不得转售。