Invitrogen

G-CSF Polyclonal Antibody, PeproTech®

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G-CSF Antibody (500-P69-1MG) in WB

Western Blot: To detect Murine G-CSF by Western Blot analysis G-CSF Polyclonal Antibody (Product # 500-P69-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine G-CSF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

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产品信息

500-P69-1MG

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.2 µg/mL

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酶联免疫吸附实验 (ELISA)

0.5-2.0 µg/mL

体外检测 (IV)

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其他PubMed (Misc)

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产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rabbit

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived Recombinant Murine G-CSF

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

0.1-1.0 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

no preservative

保存条件

-20°C

运输条件

Ambient

RRID

AB_2930708

产品详细信息

AA Sequence of recombinant protein: MVPLVTVSAL PPSLPLPRSF LLKSLEQVRK IQASGSVLLE QLCATYKLCH PEELVLLGHS LGIPKASLSG CSSQALQQTQ CLSQLHSGLC LYQGLLQALS GISPALAPTL DLLQLDVANF ATTIWQQMEN LGVAPTVQPT QSAMPAFTSA FQRRAGGVLA ISYLQGFLET ARLALHHLA.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine G-CSF. Anti-Murine G-CSF-specific antibody was purified by affinity chromatography employing an immobilized Murine G-CSF matrix.

Sandwich ELISA: To detect Murine G-CSF by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine G-CSF (500-P69Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine G-CSF.

Western Blot: To detect Murine G-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine G-CSF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

靶标信息

G-CSF (Granulocyte colony stimulating factor) is a naturally occurring cytokine that stimulates the production and antibacterial function of neutrophils and monocytes. Human G-CSF is an 18.8 kDa protein containing 175 amino acid residues, and a soluble isoform of the G-CSF receptor has been described. The pleotropic cytokine is produced by activated monocytes, macrophages, endothelial cells, fibroblasts, astrocytes, osteoblasts and bone marrow cells. G-CSF has been shown to have specific effects on the proliferation, differentiation and activation of hematopoietic cells. G-CSF is also expressed by various transformed cells such as carcinoma cells and myeloblastic leukemia cells. G-CSF is encoded by two distinct DNA sequences, resulting in a full size, high activity and a shorter, low activity isoform of G-CSF. G-CSF is highly conserved among species and has been shown to exert its biological functions through interaction with its receptor expressed on the surface of hematopoietic progenitors, neutrophilic granulocytes and certain carcinoma cell lines. Clinical use of G-CSF has been approved for several therapeutic applications, treatment of neonatal infections, therapy of acute myocardial infarction, granulocyte transfusion in patients with neutropenia, in severe infections and sepsis, therapy in chronic autoimmune neutropenia, treatment of acute myeloid leukemias, Sweet's syndrome and AIDS. Further, G-CSF has been shown to be a marker protein for different carcinomas such as bladder cancer and dysfunction of the protein has been linked to Kostmann Syndrome.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

G-CSF is a key modulator of MDSC and could be a potential therapeutic target in colitis-associated colorectal cancers.

Protein & cell

Li W,Zhang X,Chen Y,Xie Y,Liu J,Feng Q,Wang Y,Yuan W,Ma J

500-P69 was used in Western Blotting to indicate that G-CSF is a critical regulating molecule in the migration, proliferation and functional maintenance of myeloid derived suppressor cells.