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Application
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GABRD Antibody (MA5-45484) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% PFA for 15 min. Samples were incubated with GABA-A Receptor Delta monoclonal antibody (Product # MA5-45484) at 1:100 for overnight at 4°C with slow rocking, followed by AlexaFluor 488 at 1:1,000 for 1 hour at RT. Counterstain used was Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GABA-A Receptor D... View More
产品信息
MA5-45484
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Mouse
/ IgG2a
分类
Monoclonal
类型
Antibody
克隆号
N151/3
抗原
Synthetic peptide amino acids 15-34 (N-terminus) of rat GABA-A-R-Delta
偶联物
激发/发射光谱
565/576 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein G
保存液
95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA
内含物
no preservative
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2931938
产品详细信息
2 µg/mL of MA5-45484 was sufficient for detection of Delta1 GABA-A receptor in 10 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 55kDa.
靶标信息
GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the gaba/benzodiazepine receptor and opening an integral chloride channel.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: GABA(A) receptor subunit delta; GABA(A) receptor, delta; GABA-A receptor delta-subunit; GABA-A receptor, delta polypeptide; GABAA receptor delta subunit; GABAAR subunit delta; GABAARdelta; gamma-aminobutyric acid; gamma-aminobutyric acid A receptor, delta; gamma-aminobutyric acid (GABA) A receptor, delta; gamma-aminobutyric acid (GABA-A) receptor, subunit delta; gamma-aminobutyric acid A receptor, delta; Gamma-aminobutyric acid receptor subunit delta; MGC105467; MGC45284
基因别名: AI853201; EIG10; EJM7; GABAA-RD; GABRD; GEFSP5
UniProt ID: (Human) O14764, (Mouse) P22933, (Rat) P18506
Entrez Gene ID: (Human) 2563, (Mouse) 14403, (Rat) 29689
