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Invitrogen
GAD65/GAD67 Polyclonal Antibody
高级验证
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
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GAD65/GAD67 Antibody (PA1-84572) in WB
Western blot was performed using Anti-GAD65/GAD67 Polyclonal Antibody (Product # PA1-84572) and a 60 kDa band corresponding to GAD65/GAD67 was observed across tested samples. Whole cell extracts (40 µg lysate) of Mouse Brain (Lane 1), Rat Brain (Lane 2), Mouse Kidney (Lane 3), Rat Kidney (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probe... View More
产品信息
PA1-84572
应用
建议稀释比
已发表文章
产品规格
种属反应
Cat,
Human,
Mouse,
Rat
已发表种属
Not Applicable
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide sequence DFLIEEIERLGQDL from the C-terminus of GAD.
偶联物
形式
Liquid
纯化类型
Protein A
保存液
PBS
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2263114
产品详细信息
A suggested positive control for immunohistochemical applications is brain. In Western blot, a doublet at ~65/67 kDa has been observed.
Rabbit anti Glutamate Decarboxylase 1/2 antibody recognizes both molecular forms of glutamate decarboxylase, GAD1 (GAD67) and GAD2 (GAD65), the enzyme which converts glutamic acid to gamma -aminobutyric acid (GABA), the major inhibitory transmitter in the higher brain region.
靶标信息
GAD-65 and GAD-67 are members of the group II decarboxylase family of proteins and are responsible for catalyzing the rate limiting step in the production of GABA ( gamma-aminobutyric acid) from L-glutamic acid. Although both GADs are found in the brain, GAD-65 localizes to synaptic vesicle membranes in nerve terminals, while GAD-67 is distributed throughout the cell. GAD-67 is responsible for the basal levels of GABA synthesis. In the case of a heightened demand for GABA in neurotransmission, GAD-65 will transiently activate to assist in GABA production. The loss of GAD-65 is detrimental and can impair GABA neurotransmission, however the loss of GAD-67 is lethal. Due to alternative splicing, two isoforms exist for GAD-67: the predominant GAD-67 form and the minor GAD-25 form.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: 65 kDa glutamic acid decarboxylase; 67 kDa glutamic acid decarboxylase; EC 4.1.1.15; FLJ45882; GAD 65; GAD 67; GAD-65; GAD-67; Glutamate decarboxylase 1; Glutamate decarboxylase 1 (brain); glutamate decarboxylase 1 (brain, 67kDa); glutamate decarboxylase 1 variant GAD67NT; Glutamate decarboxylase 2; Glutamate decarboxylase 2 (islet); glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa); Glutamate decarboxylase 65 kDa isoform; Glutamate decarboxylase 67 kDa isoform; GLUTAMATE DECARBOXYLASE, 67 KD ISOFORM (GAD-67) (67 KD GLUTAMIC ACID DECARBOXYLASE); Glutamate decarboxylase-2 (pancreas); glutamic acid decarboxylase; glutamic acid decarboxylase 1; glutamic acid decarboxylase 2; glutamic acid decarboxylase 65
基因别名: 6330404F12Rik; CPSQ1; EP10; GAD; GAD(65); Gad-1; Gad-2; GAD1; GAD2; GAD25; GAD44; GAD65; GAD67; SCP
UniProt ID: (Human) Q99259, (Cat) P14748, (Human) Q05329, (Mouse) P48318, (Rat) P18088, (Mouse) P48320, (Rat) Q05683
Entrez Gene ID: (Human) 2571, (Cat) 493699, (Human) 2572, (Mouse) 14415, (Rat) 24379, (Mouse) 14417, (Rat) 24380
glutamate catabolic process
glutamate decarboxylation to succinate
chemical synaptic transmission
neurotransmitter secretion
gamma-aminobutyric acid biosynthetic process
protein-pyridoxal-5-phosphate linkage
neurotransmitter biosynthetic process
response to drug
carboxylic acid metabolic process
social behavior
locomotory exploration behavior
