Invitrogen

GADD45B Polyclonal Antibody

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

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5 篇参考文献

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GADD45B Antibody in Immunohistochemistry (IHC)

GADD45B Antibody in Immunohistochemistry (IHC)

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GADD45B Antibody (PA5-43160) in IHC

Immunohistochemistry analysis of human prostate cancer cells using an anti-GADD45B polyclonal antibody (Product # PA5-43160). Primary Antibody Dilution: 2 µg/mL.

GADD45B Antibody in Immunohistochemistry (IHC)

GADD45B Antibody in Immunohistochemistry (IHC)

GADD45B Antibody in Immunohistochemistry (IHC)

GADD45B Antibody in Western Blot (WB)

GADD45B Antibody in Western Blot (WB)

GADD45B Antibody in Immunocytochemistry (ICC/IF)

GADD45B Antibody in Immunocytochemistry (ICC/IF)

GADD45B Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

GADD45B Antibody in Western Blot, Immunohistochemistry (WB, IHC)

GADD45B Antibody in Western Blot (WB)

GADD45B Antibody

Product Image Gallery

GADD45B Antibody in Immunohistochemistry (IHC) — Immunohistochemistry analysis of human prostate cancer cells using an anti-GADD45B polyclonal antibody (Product # PA5-43160). Primary Antibody Dilution: 2 µg/mL.

GADD45B Antibody in Immunohistochemistry (IHC) — Immunohistochemistry analysis of human prostate cancer cells using an anti-GADD45B polyclonal antibody (Product # PA5-43160). Primary Antibody Dilution: 2 µg/mL.

GADD45B Antibody in Immunohistochemistry (IHC) — Immunohistochemistry analysis of human prostate cancer cells using an anti-GADD45B polyclonal antibody (Product # PA5-43160). Primary Antibody Dilution: 2 µg/mL.

GADD45B Antibody in Western Blot (WB) — Western blot analysis of human fetal lung cell lysate using an anti-GADD45B polyclonal antibody (Product # PA5-43160).

GADD45B Antibody in Western Blot (WB) — Western blot analysis of human HepG2 cell lysate using an anti-GADD45B polyclonal antibody (Product # PA5-43160).

GADD45B Antibody in Immunocytochemistry (ICC/IF) — Figure 3 Effects of GADD45B knockdown on renal tubular epithelial-mesenchymal transition (EMT) and apoptosis in HK-2 cells under high glucose (HG) conditions. (A) Representative images of immunofluorescence staining of GADD45B in HK-2 cells. Original magnification = 200. Scale bar = 200 mum. (B) Western blot bands showing E-cadherin, Vimentin, and alpha-smooth muscle actin (alpha-SMA) protein expression in HK-2 cells. (C) Quantitative analysis of (B) , N = 3. (D) Western blot bands showing Bax, Bcl2, and cleaved Caspase 3 protein expression in HK-2 cells. (E) Quantitative analysis of (D) , N = 3. The data are presented as the mean +- SD. * p < 0.05.

GADD45B Antibody in Immunocytochemistry (ICC/IF) — Figure 4 Effects of GADD45B overexpression on renal tubular EMT and apoptosis in HK-2 cells. (A) Representative images of immunofluorescence staining of GADD45B in HK-2 cells. Original magnification = 200. Scale bar = 200 mum. (B) Western blot bands showing E-cadherin, Vimentin, and alpha-SMA protein expression in HK-2 cells. (C) Quantitative analysis of (B) , N = 4. (D) Western blot bands showing Bax, Bcl2, and cleaved Caspase 3 protein expression in HK-2 cells. (E) Quantitative analysis of (D), N = 4. The data are presented as the mean +- SD. * p < 0.05.

GADD45B Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF) — Fig. 4. Changes of neuroprotective molecules after global ischemia. (A) Wnt family mRNA expression showing time-dependent alterations in ischemia hippocampal CA1 regions as determined by quantitative real time-polymerase chain reaction (qRT-PCR). (B) Growth arrest and DNA-damage-inducible protein 45 beta (Gadd45b) mRNA expression quantified by qRT-PCR. (C) Representative immunoblot images of Gadd45b in hippocampal CA1 with complementary quantifications normalized by tubulin (n=7 for sham, 4 for ischemia 24- and 48-hour animals). Values represent mean +-standard error of the mean (*P <0.05. **P <0.01. ***P <0.001. ****P<0.0001). (D) Confocal imaging depicting Gadd45b (red), counterstained with 4'-6-diamidino-2-phenylindole (DAPI) (blue). Scale bar is 20 um.

GADD45B Antibody in Western Blot, Immunohistochemistry (WB, IHC) — Figure 2 The expression of growth arrest and DNA damage-inducible beta (GADD45B) was increased in the kidneys of db/db mice and HG-induced HK-2 cells. (A) Heat map of the 1,194 differentially expressed genes (the logarithm of the fold change to base 2 > 2) and the right part is the enlarged view containing GADD45B. (B) The logarithm of the fold change to base 2 of GADD45B expression was determined by RNA sequencing analysis. (C) Real-time PCR (RT-PCR) analysis of GADD45B messenger RNA (mRNA) expression in kidney samples, N = 9. (D) Representative images of immunohistochemical staining of GADD45B in kidney sections. Original magnification = 400. Scale bar = 100 mum. (E,F) Western blot bands and quantitative analysis of GADD45B protein expression in kidney samples, N = 6. (G,H) RT-PCR analysis of GADD45B mRNA expression in HK-2 cells, N = 3. (I-L) Western blot bands and quantitative analysis of GADD45B protein expression in HK-2 cells, N = 3. The data are presented as the mean +- SD. * p < 0.05.

GADD45B Antibody in Western Blot (WB) — Fig. 5. Knockdown of growth arrest and DNA-damage-inducible protein 45 beta (Gadd45b) accelerates neuronal cell death and mitochondrial dysfunction. (A) Validation of knockdown efficiency for Gadd45b shRNA in cortical neurons by immunoblotting. (B) Summary data of lactate dehydrogenase (LDH) activity assay for cortical neurons treated with 50uM H 2 O 2 (2 hours) in the presence of nontargeting (NT) or Gadd45b shRNA. LDH activity values are normalized to the value of cells exposed to 400muM H 2 O 2 (100%, FK; full kill) after subtracting the value of control cells (0%). (C) Representative immunoblot images showing Bcl-2. (D) Bax expression level in cortical neurons treated with 50uM H 2 O 2 (2 hours) in the presence of NT or Gadd45b shRNA. (E) Quantitative real timepolymerase chain reaction data showing brain-derived neurotrophic factor (BDNF) mRNA expression at indicated time periods after ischemia. (F) Representative immunoblot images Gadd45b in hippocampal CA1 and summary data. (n=7 for sham, 4 for ischemia 24- and 48-hour animals). Values represent mean+-standard error of themean. *P<0.05. **P<0.01. ***P<0.001.

GADD45B Antibody — Figure 5. Knockdown of growth arrest and DNA-damage-inducible protein 45 beta (Gadd45b) accelerates neuronal cell death and mitochondrial dysfunction. (A) Validation of knockdown efficiency for Gadd45b shRNA in cortical neurons by immunoblotting. (B) Summary data of lactate dehydrogenase (LDH) activity assay for cortical neurons treated with 50µM H2O2 (2 hours) in the presence of nontargeting (NT) or Gadd45b shRNA. LDH activity values are normalized to the value of cells exposed to 400µM H2O2 (100%, FK; full kill) after subtracting the value of control cells (0%). (C) Representative immunoblot images showing Bcl-2. (D) Bax expression level in cortical neurons treated with 50µM H2O2 (2 hours) in the presence of NT or Gadd45b shRNA. (E) Quantitative real timepolymerase chain reaction data showing brain-derived neurotrophic factor (BDNF) mRNA expression at indicated time periods after ischemia. (F) Representative immunoblot images Gadd45b in hippocampal CA1 and summary data. (n=7 for sham, 4 for ischemia 24- and 48-hour animals). Values represent mean±standard error of themean. *P<0.05. **P<0.01. ***P<0.001. {KD}

产品信息

PA5-43160

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.2-1 µg/mL

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免疫组化 (IHC)

Assay-Dependent

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免疫细胞化学 (ICC/IF)

-

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产品规格

种属反应

Human, Mouse

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide directed towards the middle region of human GADD45B

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS with 2% sucrose

内含物

0.09% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

RRID

AB_2610079

产品详细信息

Peptide sequence: FCCDNDINIV RVSGMQRLAQ LLGEPAETQG TTEARDLHCL LVTNPHTDAW

Sequence homology: Cow: 100%; Dog: 100%; Guinea Pig: 92%; Horse: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rat: 100%

靶标信息

Cellcycle progression issubject to arrest at G1 and G2 checkpointsin response to DNA damage, presumablyto allow time for DNA repair prior to entryinto S andMphase, respectively. The p53 tumorsuppressor isrequired for one such G1 checkpoint and functionsto upregulate expression of GADD 45 and p21. GADD 45 binds both Cdks and PCNA, a protein involved in DNA replication and repair. GADD 45 stimulates DNA excision repair in vitro and inhibits entry ofcellsinto S phase. Thus, it has been suggested that GADD 45 mayserve as a link between the p53-dependentcellcycle checkpoint and DNA repair. GADD 45-like proteins, GADD 45 beta and GADD 45 gamma, have been shown to be induced by environmentalstresses. GADD 45 beta and GADD 45 gamma are thought to induce p38/JNK activation via MEKK4 activation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

GADD45B Promotes Glucose-Induced Renal Tubular Epithelial-Mesenchymal Transition and Apoptosis via the p38 MAPK and JNK Signaling Pathways.

Frontiers in physiology

Xue M,Sun H,Xu R,Wang Y,Guo J,Li X,Cheng Y,Xu C,Tang C,Sun B,Chen L

PA5-43160 was used in Western Blot, Immunohistochemistry, Immunocytochemistry to determine that GADD45B was significantly upregulated in diabetic kidneys, which was accompanied by renal tubular epithelial-mesenchymal transition (EMT) and apoptosis, as well as elevated MAPK pathway activation.

Tue Oct 06 00:00:00 EDT 2020

GADD45B Promotes Glucose-Induced Renal Tubular Epithelial-Mesenchymal Transition and Apoptosis via the p38 MAPK and JNK Signaling Pathways.

Frontiers in physiology

Xue M,Sun H,Xu R,Wang Y,Guo J,Li X,Cheng Y,Xu C,Tang C,Sun B,Chen L

PA5-43160 was used in Western Blot, Immunohistochemistry, Immunocytochemistry to determine that GADD45B was significantly upregulated in diabetic kidneys, which was accompanied by renal tubular epithelial-mesenchymal transition (EMT) and apoptosis, as well as elevated MAPK pathway activation.

Tue Oct 06 00:00:00 EDT 2020

GADD45B Promotes Glucose-Induced Renal Tubular Epithelial-Mesenchymal Transition and Apoptosis via the p38 MAPK and JNK Signaling Pathways.

Frontiers in physiology

Xue M,Sun H,Xu R,Wang Y,Guo J,Li X,Cheng Y,Xu C,Tang C,Sun B,Chen L

PA5-43160 was used in Western Blot, Immunohistochemistry, Immunocytochemistry to determine that GADD45B was significantly upregulated in diabetic kidneys, which was accompanied by renal tubular epithelial-mesenchymal transition (EMT) and apoptosis, as well as elevated MAPK pathway activation.

Tue Oct 06 00:00:00 EDT 2020

GADD45B Promotes Glucose-Induced Renal Tubular Epithelial-Mesenchymal Transition and Apoptosis via the p38 MAPK and JNK Signaling Pathways.

Frontiers in physiology

Xue M,Sun H,Xu R,Wang Y,Guo J,Li X,Cheng Y,Xu C,Tang C,Sun B,Chen L

PA5-43160 was used in Western Blot, Immunohistochemistry, Immunocytochemistry to determine that GADD45B was significantly upregulated in diabetic kidneys, which was accompanied by renal tubular epithelial-mesenchymal transition (EMT) and apoptosis, as well as elevated MAPK pathway activation.

Tue Oct 06 00:00:00 EDT 2020

GADD45B Promotes Glucose-Induced Renal Tubular Epithelial-Mesenchymal Transition and Apoptosis via the p38 MAPK and JNK Signaling Pathways.

Frontiers in physiology

Xue M,Sun H,Xu R,Wang Y,Guo J,Li X,Cheng Y,Xu C,Tang C,Sun B,Chen L

PA5-43160 was used in Western Blot, Immunohistochemistry, Immunocytochemistry to determine that GADD45B was significantly upregulated in diabetic kidneys, which was accompanied by renal tubular epithelial-mesenchymal transition (EMT) and apoptosis, as well as elevated MAPK pathway activation.

Tue Oct 06 00:00:00 EDT 2020

GADD45B Promotes Glucose-Induced Renal Tubular Epithelial-Mesenchymal Transition and Apoptosis via the p38 MAPK and JNK Signaling Pathways.

Frontiers in physiology

Xue M,Sun H,Xu R,Wang Y,Guo J,Li X,Cheng Y,Xu C,Tang C,Sun B,Chen L

PA5-43160 was used in Western Blot, Immunohistochemistry, Immunocytochemistry to determine that GADD45B was significantly upregulated in diabetic kidneys, which was accompanied by renal tubular epithelial-mesenchymal transition (EMT) and apoptosis, as well as elevated MAPK pathway activation.

Tue Oct 06 00:00:00 EDT 2020

Gadd45b Acts as Neuroprotective Effector in Global Ischemia-Induced Neuronal Death.

International neurourology journal

Cho CH,Byun HR,Jover-Mengual T,Pontarelli F,Dejesus C,Cho AR,Zukin RS,Hwang JY

Published figure using GADD45B polyclonal antibody (Product # PA5-43160) in Immunocytochemistry

Fri Feb 01 00:00:00 EST 2019

Gadd45b Acts as Neuroprotective Effector in Global Ischemia-Induced Neuronal Death.

International neurourology journal

Cho CH,Byun HR,Jover-Mengual T,Pontarelli F,Dejesus C,Cho AR,Zukin RS,Hwang JY

Published figure using GADD45B polyclonal antibody (Product # PA5-43160) in Immunocytochemistry

Fri Feb 01 00:00:00 EST 2019

生物信息学

蛋白别名: DKFZP566B133; Growth arrest and DNA damage-inducible protein GADD45 beta; growth arrest and DNA-damage-inducible protein GADD45 beta; growth arrest and DNA-damage-inducible, beta; myeloid differentiation primary response gene 118; Myeloid differentiation primary response protein MyD118; Negative growth regulatory protein MyD118

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基因别名: AI323528; GADD45B; GADD45BETA; MYD118

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UniProt ID: (Human) O75293, (Mouse) P22339

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Entrez Gene ID: (Human) 4616, (Mouse) 17873

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protein binding

参与通路

activation of MAPKKK activity activation of MAPKK activity negative regulation of protein kinase activity apoptotic process response to stress multicellular organism development cell differentiation positive regulation of apoptotic process positive regulation of JNK cascade regulation of cell cycle positive regulation of p38MAPK cascade