Invitrogen

GAPDH Recombinant Rabbit Monoclonal Antibody (4A9L6)

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

4 篇参考文献

查看其他79个GAPDH抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (12)
高级验证 (1)

GAPDH Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 13

GAPDH Antibody (MA5-35235) in ICC/IF

Confocal imaging (Immunocytochemistry) of GAPDH in HeLa cells. Samples were incubated with GAPDH Monoclonal antibody (Product # MA5-35235) using a dilution of 1:100 (Red). The cells were counterstained with alpha Tubulin Mouse mAb (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

GAPDH Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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GAPDH Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with GAPDH siRNA and decrease in signal intensity was observed in Western Blot application using GAPDH Recombinant Rabbit Monoclonal Antibody (ARC0205) (Product # MA5-35235). {KD}

产品信息

MA5-35235

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:50,000-1:200,000

查看4篇已发表文章 4篇已发表文章

免疫组化（石蜡） (IHC (P))

1:500-1:1,000

免疫细胞化学 (ICC/IF)

1:50-1:200

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Algae, Human, Mouse

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

4A9L6

抗原

Recombinant fusion protein containing a sequence corresponding to amino acids 4-335 of human GAPDH (NP_0020372)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.10 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

内含物

0.05% ProClin 300

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

RRID

AB_2849138

产品详细信息

Immunogen sequence: VKVGVNGFGR IGRLVTRAAF NSGKVDIVAI NDPFIDLNYM VYMFQYDSTH GKFHGTVKAE NGKLVINGNP ITIFQERDPS KIKWGDAGAE YVVESTGVFT TMEKAGAHLQ GGAKRVIISA PSADAPMFVM GVNHEKYDNS LKIISNASCT TNCLAPLAKV IHDNFGIVEG LMTTVHAITA TQKTVDGPSG KLWRDGRGAL QNIIPASTGA AKAVGKVIPE LNGKLTGMAF RVPTANVSVV DLTCRLEKPA KYDDIKKVVK QASEGPLKGI LGYTEHQVVS SDFNSDTHSS TFDAGAGIAL NDHFVKLISW YDNEFGYSNR VVDLMAHMAS KE

靶标信息

GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme commonly known to be involved in glycolysis. GAPDH exists as a tetramer of identical 37-kDa subunits and catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Apart from playing a key role in glycolysis, GAPDH is ubiquitously expressed and displays other activities unrelated to its glycolytic function. GAPDH is reported to be involved in the processes of DNA replication, DNA repair, nuclear RNA export, membrane fusion and microtubule bundling. Studies provide evidence of GAPDH playing an essential part in gene expression observed in apoptosis and as part of the cellular phenotype of age-related neurodegenerative diseases. Further, GAPDH is involved in other cellular processes ranging from membrane fusion, and neuronal apoptosis in cancer. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease (AD), and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease. Associations between GAPDH, actin and tubulin have also be reported. Since GAPDH is expressed at high levels in most tissues, it is useful as protein loading control in Western Blot analysis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Identification of Flavone Derivative Displaying a 4'-Aminophenoxy Moiety as Potential Selective Anticancer Agent in NSCLC Tumor Cells.

Molecules (Basel, Switzerland)

Mobbili G,Romaldi B,Sabbatini G,Amici A,Marcaccio M,Galeazzi R,Laudadio E,Armeni T,Minnelli C

MA5-35235 was used in Western Blotting to investigate the cytotoxicity of five heterocyclic derivatives in vitro in two models of human non-small cell lung cancer (NSCLC) cells (A549 and NCI-H1975).

Wed Apr 05 00:00:00 EDT 2023

Binding characterization of anthraquinone derivatives by stabilizing G-quadruplex DNA leads to an anticancerous activity.

Molecular therapy. Nucleic acids

Dey A,Pandav K,Nath M,Barthwal R,Prasad R

MA5-35235 was used in Western Blotting to focus on how ligand binding to telomere sequences induces oxidative stress and inhibits the growth of malignant cells.

Tue Dec 13 00:00:00 EST 2022

miR-133a-3p Regulates Hepatocellular Carcinoma Progression Through Targeting CORO1C.

Cancer management and research

Han S,Ding X,Wang S,Xu L,Li W,Sun W

MA5-35235 was used in Western Blot to reveal a novel mechanism of miR-133a-3p in regulating HCC progression and provided evidence that miR-133a-3p functions as a tumor suppressor in HCC.

Sun Apr 17 00:00:00 EDT 2022

Protective Effect of a Fucose-Rich Fucoidan Isolated from Saccharina japonica against Ultraviolet B-Induced Photodamage In Vitro in Human Keratinocytes and In Vivo in Zebrafish.

Marine drugs

Su W,Wang L,Fu X,Ni L,Duan D,Xu J,Gao X

MA5-35235 was used in Western Blot to indicate that purified fucoidan significantly reduces intracellular reactive oxygen species levels and improves the viability of UVB-irradiated HaCaT cells.

Mon Jun 15 00:00:00 EDT 2020

生物信息学

蛋白别名: 38 kDa BFA-dependent ADP-ribosylation substrate; aging-associated gene 9 protein; BARS-38; EC 1.2.1.12; epididymis secretory protein Li 278; epididymis secretory sperm binding protein Li 162eP; GAPDH; GAPDH-2; Glyceraldehyde-3-phosphate dehydrogenase; glyceraldehyde-3-phosphate dehydrogenase type 2; Glyceraldehyde-3-phosphate dehydrogenase, testis-specific; HGNC:4141; MGC88685; Peptidyl-cysteine S-nitrosylase GAPDH; Spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2; Spermatogenic glyceraldehyde-3-phosphate dehydrogenase

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基因别名: BARS-38; CDABP0047; G3PD; GAPD; Gapd-s; GAPD2; GAPDH; GAPDH-2; GAPDH2; GAPDHS; GAPDS; HEL-S-162eP; HEL-S-278; HSD-35; HSD35; OK/SW-cl.12