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GAPDH Loading Control Antibody (MA5-15738-D680) in ICC/IF
Immunofluorescent analysis of GAPDH (red) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a DyLight 680-conjugated GAPDH monoclonal antibody (Product # MA5-15738-D680) at a dilution of 1:50 for at least 1 hour at room temperature... View More
产品信息
MA5-15738-D680
应用
建议稀释比
已发表文章
产品规格
种属反应
Bacteria,
Chicken,
Hamster,
Human,
Insect,
Mouse,
Rabbit,
Rat,
Yeast
已发表种属
Human,
Mouse,
Rat
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
GA1R
抗原
Recombinant GAPDH
偶联物
DyLight™ 680
激发/发射光谱
676/705 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS with proprietary stabilizer
内含物
0.02% sodium azide
保存条件
4° C, do not freeze
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2537657
产品详细信息
MA5-15738-D680 has successfully been used in immunofluorescence and Western blot applications. DyLight 680 has an excitation/emission of 692/712 nm.
靶标信息
GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme commonly known to be involved in glycolysis. GAPDH exists as a tetramer of identical 37-kDa subunits and catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Apart from playing a key role in glycolysis, GAPDH is ubiquitously expressed and displays other activities unrelated to its glycolytic function. GAPDH is reported to be involved in the processes of DNA replication, DNA repair, nuclear RNA export, membrane fusion and microtubule bundling. Studies provide evidence of GAPDH playing an essential part in gene expression observed in apoptosis and as part of the cellular phenotype of age-related neurodegenerative diseases. Further, GAPDH is involved in other cellular processes ranging from membrane fusion, and neuronal apoptosis in cancer. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease (AD), and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease. Associations between GAPDH, actin and tubulin have also be reported. Since GAPDH is expressed at high levels in most tissues, it is useful as protein loading control in Western Blot analysis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: 38 kDa BFA-dependent ADP-ribosylation substrate; aging-associated gene 9 protein; BARS-38; EC 1.2.1.12; epididymis secretory protein Li 278; epididymis secretory sperm binding protein Li 162eP; GAPDH; GAPDH-2; glceraldehyde-3-phosphate dehydrogenase; Glyceraldehyde-3-phosphate dehydrogenase; glyceraldehyde-3-phosphate dehydrogenase type 2; glyceraldehyde-3-phosphate dehydrogenase, spermatogenic; Glyceraldehyde-3-phosphate dehydrogenase, testis-specific; HGNC:4141; MGC88685; multifunctional protein, glycolytic enzyme; Peptidyl-cysteine S-nitrosylase GAPDH; Spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2; Spermatogenic glyceraldehyde-3-phosphate dehydrogenase; unnamed protein product
基因别名: BARS-38; CDABP0047; G3PD; GAPD; Gapd-s; GAPD2; GAPDH; GAPDH-2; GAPDH2; GAPDHS; GAPDS; HEL-S-162eP; HEL-S-278; HSD-35; HSD35; I79_001391; KNC-NDS6; OK/SW-cl.12
glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
protein binding
microtubule binding
peptidyl-cysteine S-nitrosylase activity
identical protein binding
NADP binding
NAD binding
aspartic-type endopeptidase inhibitor activity
disordered domain specific binding
molecular_function
oxidoreductase activity
oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
dehydrogenase
microtubule cytoskeleton organization
gluconeogenesis
regulation of macroautophagy
negative regulation of translation
peptidyl-cysteine S-trans-nitrosylation
protein stabilization
neuron apoptotic process
canonical glycolysis
cellular response to interferon-gamma
nuclear membrane fusion
glycolytic process
negative regulation of endopeptidase activity
negative regulation of apoptotic process
positive regulation of cytokine secretion
defense response to fungus
killing by host of symbiont cells
positive regulation by organism of apoptotic process in other organism involved in symbiotic interaction
oxidation-reduction process
antimicrobial humoral immune response mediated by antimicrobial peptide
spermatid development
sperm motility
positive regulation of glycolytic process
carbohydrate metabolic process
apoptotic process
multicellular organism development
response to ammonium ion
regulation of translation
glucose metabolic process
