Invitrogen

GARP Monoclonal Antibody (YGIC86), PE, eBioscience™

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GARP Antibody (12-9891-82) in Flow

BALB/c splenocytes were stimulated with Anti-Mouse CD3e Functional Grade Purified (Product # 16-0031-82), Anti-Mouse CD28 Functional Grade Purified (Product # 16-0281-82), and Mouse IL-2 Recombinant Protein (Product # 14-8021-64) for two days. To exclude dead cells from analysis, cells were stained with Fixable Viability Dye eFluor® 450 (Product # 65-0863-14), then cells were surface stained with Anti-Mouse CD4 FITC (Product # 11-0042-82) and 0.25 µg of Rat IgG2a kappa Isotype Control PE (Product # 12-4321-80) (left) or 0.25 µg of Anti-Mou... View More

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产品信息

12-9891-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.25 µg/test

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产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

YGIC86

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_1963598

产品详细信息

Description: The YGIC86 monoclonal antibody reacts with mouse Glycoprotein A Repetitions Predominant (GARP, also known as Lrrc32 or Garpin). GARP is an approximately 80 kDa glycoprotein that is expressed on the cell surface. Using northern blot, RT-PCR or microarray analyses, the expression of GARP has been reported in placenta, lung, kidney, heart, ovary, liver, skeletal muscle, and pancreas. Protein expression has been observed on megakaryocytes, platelets and activated regulatory T (Treg) cells. The expression of GARP on Treg cells has been reported to be necessary for the suppressive function of Treg cells, possibly related to its role as a cell surface receptor for LAP/TGF beta.

When looking for expression of GARP on platelets, it is recommended to use the Foxp3 Staining Buffer Set (Product # 00-5523) to fix the platelets before staining with this YGIC86 monoclonal antibody. Fixation/permeabilization is not necessary when staining Treg cells.

YGIC86 does not cross-react to rat GARP.

Applications Reported: This YGIC86 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This YGIC86 antibody has been tested by flow cytometric analysis of stimulated mouse spleen cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

GARP, also known as leucine rich repeat containing 32, is a type I membrane protein with twenty-two leucine-rich repeats and a short intracytoplasmic region. GARP was identified based on the analysis of gene expression profiling of Treg and Th cells following TCR stimulation. Since GARP is expressed in CD4+, CD25+, FoxP3+ cells (Tregs), it may be a candidate for cell surface marker for Treg cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Fine-tuning of antigen-specific immune responses by regulatory T cells activated via antigen recognition-independent and humoral factor-dependent mechanisms.

Scandinavian journal of immunology

Kojima H,Kashiwakura Y,Kanno Y,Hashiguchi M,Kobata T

12-9891-82 was used in Flow cytometry/Cell sorting to suggest that Tregs are activated by cytokines, mainly IL-2, released from T cells that are activated by a specific antigen.

Tue Jun 01 00:00:00 UTC 2021

A distal enhancer at risk locus 11q13.5 promotes suppression of colitis by T_reg cells.

Nature

Nasrallah R,Imianowski CJ,Bossini-Castillo L,Grant FM,Dogan M,Placek L,Kozhaya L,Kuo P,Sadiyah F,Whiteside SK,Mumbach MR,Glinos D,Vardaka P,Whyte CE,Lozano T,Fujita T,Fujii H,Liston A,Andrews S,Cozzani A,Yang J,Mitra S,Lugli E,Chang HY,Unutmaz D,Trynka G,Roychoudhuri R

12-9891 was used in Flow cytometry/Cell sorting to provide a mechanistic basis for association of the 11q13.5 risk locus with immune-mediated diseases and identify GARP as a potential target in their therapy.

Wed Jul 01 00:00:00 UTC 2020

Heparin affects the induction of regulatory T cells independent of anti-coagulant activity and suppresses allogeneic immune responses.

Clinical and experimental immunology

Kashiwakura Y,Kojima H,Kanno Y,Hashiguchi M,Kobata T

12-9891-82 was used in Flow Cytometry to analyse the effect of heparin on regulatory T cells in allogenic immune responses.

Thu Oct 01 00:00:00 UTC 2020

Intranasal immunization with heat shock protein 60 induces CD4(+) CD25(+) GARP(+) and type 1 regulatory T cells and inhibits early atherosclerosis.

Clinical and experimental immunology

Zhong Y,Tang H,Wang X,Zeng Q,Liu Y,Zhao XI,Yu K,Shi H,Zhu R,Mao X

Published figure using GARP monoclonal antibody (Product # 12-9891-82) in Flow Cytometry

Tue Mar 01 00:00:00 UTC 2016

Mesenchymal stromal cells express GARP/LRRC32 on their surface: effects on their biology and immunomodulatory capacity.

Stem cells (Dayton, Ohio)

Carrillo-Galvez AB,Cobo M,Cuevas-Ocaña S,Gutiérrez-Guerrero A,Sánchez-Gilabert A,Bongarzone P,García-Pérez A,Muñoz P,Benabdellah K,Toscano MG,Martín F,Anderson P

12-9891 was used in Flow cytometry/Cell sorting to show that human and mouse mesenchymal stromal cells express glycoprotein A repetitions predominant, which presents LAP/TGF-β1 on their cell surface.

Thu Jan 01 00:00:00 UTC 2015

Mesenchymal stromal cells express GARP/LRRC32 on their surface: effects on their biology and immunomodulatory capacity.

Stem cells (Dayton, Ohio)

Carrillo-Galvez AB,Cobo M,Cuevas-Ocaña S,Gutiérrez-Guerrero A,Sánchez-Gilabert A,Bongarzone P,García-Pérez A,Muñoz P,Benabdellah K,Toscano MG,Martín F,Anderson P

Thu Jan 01 00:00:00 UTC 2015

Identification and characterization of latency-associated peptide-expressing γδ T cells.

Nature communications

Rezende RM,da Cunha AP,Kuhn C,Rubino S,M'Hamdi H,Gabriely G,Vandeventer T,Liu S,Cialic R,Pinheiro-Rosa N,Oliveira RP,Gaublomme JT,Obholzer N,Kozubek J,Pochet N,Faria AMC,Weiner HL

12-9891 was used in Flow cytometry/Cell sorting to describe a subset of regulatory γδ T cells that express the latency-associated peptide, a membrane-bound TGF-β1.

Tue Dec 08 00:00:00 UTC 2015

Butyrophilin-like 2 modulates B7 costimulation to induce Foxp3 expression and regulatory T cell development in mature T cells.

Journal of immunology (Baltimore, Md. : 1950)

Swanson RM,Gavin MA,Escobar SS,Rottman JB,Lipsky BP,Dube S,Li L,Bigler J,Wolfson M,Arnett HA,Viney JL

12-9891 was used in Flow cytometry/Cell sorting to investigate the mechanism by which butyrophilin-like 2 modulates B7 costimulation and regulatory T cell development.

Fri Mar 01 00:00:00 UTC 2013

Identification and expansion of highly suppressive CD8(+)FoxP3(+) regulatory T cells after experimental allogeneic bone marrow transplantation.

Blood

Robb RJ,Lineburg KE,Kuns RD,Wilson YA,Raffelt NC,Olver SD,Varelias A,Alexander KA,Teal BE,Sparwasser T,Hammerling GJ,Markey KA,Koyama M,Clouston AD,Engwerda CR,Hill GR,MacDonald KP

12-9891 was used in Flow cytometry/Cell sorting to investigate the role of Foxp3(+) T cells in allogenic bone marrow transplant.

Thu Jun 14 00:00:00 UTC 2012

Murine CD4⁺CD25⁻ cells activated in vitro with PMA/ionomycin and anti-CD3 acquire regulatory function and ameliorate experimental colitis in vivo.

BMC gastroenterology

Majowicz A,van der Marel S,te Velde AA,Meijer SL,Petry H,van Deventer SJ,Ferreira V

12-9891 was used in Flow cytometry/Cell sorting to identify a new method to generate in vitro iTreg cells (TregPMA cells) with demonstrated functionality in vivo.

Mon Dec 03 00:00:00 UTC 2012

Overexpression of TGF-ß 1 gene induces cell surface localized glucose-regulated protein 78-associated latency-associated peptide/TGF-ß.

Journal of immunology (Baltimore, Md. : 1950)

Oida T,Weiner HL

Published figure using GARP monoclonal antibody (Product # 12-9891-82) in Flow Cytometry

Wed Sep 15 00:00:00 UTC 2010

TGF-β induces surface LAP expression on murine CD4 T cells independent of Foxp3 induction.

PloS one

Oida T,Weiner HL

Published figure using GARP monoclonal antibody (Product # 12-9891-82) in Flow Cytometry

Wed Nov 24 00:00:00 UTC 2010

生物信息学

蛋白别名: GARP; Garpin; Glycoprotein A repetitions predominant; Leucine-rich repeat-containing protein 32; Transforming growth factor beta activator LRRC32

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基因别名: AI426318; D11S833Eh; D7H11S833E; EG434215; Garp; Lrrc32

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UniProt ID: (Mouse) G3XA59

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Entrez Gene ID: (Mouse) 434215

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功能

transmembrane signal receptor

参与通路

positive regulation of gene expression negative regulation of activated T cell proliferation negative regulation of cytokine secretion