OriGene

GATA4 Monoclonal Antibody (OTI1H8), TrueMAB™

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抗体检测数据 (2)

GATA4 Antibody in Western Blot (WB)

GATA4 Antibody in Western Blot (WB)

图: 1 / 2

GATA4 Antibody (CF500253) in WB

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GATA4 (RC210945, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-GATA4. Positive lysates LY419558 (100 µg) and LC419558 (20 µg) can be purchased separately from OriGene.

GATA4 Antibody in Western Blot (WB)

GATA4 Antibody in Western Blot (WB)

Product Image Gallery

GATA4 Antibody in Western Blot (WB) — HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GATA4 (RC210945, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-GATA4. Positive lysates LY419558 (100 µg) and LC419558 (20 µg) can be purchased separately from OriGene.

GATA4 Antibody in Western Blot (WB) — Figure from citation: Western Blot of GATA4 protein level by using anti-GATA4 antibody in hESCs. View Citation

产品信息

CF500253

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

-

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG2b

分类

Monoclonal

类型

Antibody

克隆号

OTI1H8

抗原

Recombinant fragment expressed in E.coli corresponding to amino acids 296-442of human GATA4

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.3, with 8% trehalose

内含物

no preservative

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

靶标信息

GATA4 is a member of the GATA family of zinc-finger transcription factors. Members of this family recognize the GATA motif which is present in the promoters of many genes. This protein is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function. Mutations in the gene encoding GATA4 have been associated with cardiac septal defects.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: GAT4; GATA-binding factor 4; MGC126629; Transcription factor GATA-4

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基因别名: ASD2; GATA4; TACHD; TOF; VSD1

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UniProt ID: (Human) P43694

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Entrez Gene ID: (Human) 2626

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RNA polymerase II regulatory region sequence-specific DNA binding transcription factor activity, RNA polymerase II transcription factor binding RNA polymerase II transcription factor binding enhancer sequence-specific DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding transcription coactivator activity protein binding transcription factor binding zinc ion binding protein kinase binding activating transcription factor binding sequence-specific DNA binding transcription regulatory region DNA binding NFAT protein binding co-SMAD binding DNA-binding transcription factor

参与通路

heart looping endocardial cushion development cardiac ventricle morphogenesis cardiac right ventricle morphogenesis ventricular septum development atrial septum primum morphogenesis atrial septum secundum morphogenesis regulation of transcription, DNA-templated transcription from RNA polymerase II promoter cell-cell signaling endoderm development blood coagulation male gonad development response to mechanical stimulus negative regulation of autophagy positive regulation of vascular endothelial growth factor production positive regulation of BMP signaling pathway response to vitamin A embryonic heart tube anterior/posterior pattern specification response to drug cell fate commitment positive regulation of angiogenesis positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter cell development embryonic foregut morphogenesis anatomical structure formation involved in morphogenesis positive regulation of cardioblast differentiation transdifferentiation atrial septum morphogenesis intestinal epithelial cell differentiation cell growth involved in cardiac muscle cell development cellular response to glucose stimulus regulation of cardiac muscle cell contraction