Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
图: 1 / 9
GCLM Antibody (MA5-32783) in ICC/IF
Immunocytochemistry analysis of GCLM with A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS (10 min, room temp) and blocked with 1% Blocker BSA (15 min, room temp), incubated with recombinant monoclonal GCLM (Product # MA5-32783) at a dilution of 1:50 for 1 hour at room temperature, washed with PBS, followed by Alexa Fluor 488 Goat anti-Rabbit IgG at 1:1,000 dilution. DAPI was used to stain the cell nuclei (blue).
产品信息
MA5-32783
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Mouse
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
JM39-61
抗原
Recombinant protein within Human GCLM aa 225-274
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2800360
产品详细信息
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
靶标信息
Gamma-glutamylcysteine synthetase (gamma-GCS) is the rate limiting enzyme for glutathione (L-gamma-glutamyl-L-cysteinylglycine, GSH) synthesis. GSH is ubiquitous in mammalian cells as a vital intra- and extracellular protective antioxidant. Gamma-GCS is a heterodimer of a heavy catalytic subunit and a light regulatory subunit that is responsive to inflammation, phenolic ntioxidants, heat shock, oxidants and cytokines.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: gamma glutamylcysteine synthetase; Gamma-ECS regulatory subunit; Gamma-glutamylcysteine synthetase regulatory subunit; GCS light chain; glutamate cysteine ligase (gamma-glutamylcysteine synthetase), regulatory; Glutamate--cysteine ligase modifier subunit; Glutamate--cysteine ligase regulatory subunit; glutamate-cysteine ligase (gamma-glutamylcysteine synthetase), regulatory (30.8kD); glutamate-cysteine ligase modifier subunit; glutamate-cysteine ligase modifier subunit delta2 alternative splicing; glutamate-cysteine ligase regulatory protein; glutamate-cysteine ligase, modifier subunit; GSC light chain; null
基因别名: AI649393; GCLM; Gcmc; GLCLR
UniProt ID: (Human) P48507, (Mouse) O09172, (Rat) P48508
Entrez Gene ID: (Human) 2730, (Mouse) 14630, (Rat) 29739
sulfur amino acid metabolic process
cysteine metabolic process
glutamate metabolic process
glutathione biosynthetic process
response to oxidative stress
apoptotic mitochondrial changes
positive regulation of glutamate-cysteine ligase activity
response to drug
negative regulation of neuron apoptotic process
regulation of blood vessel size
response to nitrosative stress
regulation of mitochondrial depolarization
negative regulation of extrinsic apoptotic signaling pathway
glutathione metabolic process
regulation of catalytic activity
