Invitrogen

GFAP Polyclonal Antibody

高级验证

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

2个已发表图片

5 篇参考文献

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说明书

实验方案

技术支持

说明书

实验方案

技术支持

GFAP Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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GFAP Antibody (PA5-18598) in IHC (P)

Immunohistochemistry analysis of GFAP in human brain, cortex, astrocytes. Samples were incubated with GFAP polyclonal antibody (Product # PA5-18598) using a dilution of 5 µg/mL. Formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

GFAP Antibody in Immunocytochemistry (ICC/IF)

GFAP Antibody in Immunohistochemistry (IHC)

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GFAP Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target in tissues owing to their inherent genetic constitution. Differential basal expression of GFAP was observed across the tissues where the brain samples tested positive for GFAP using product (Product # PA5-18598) in western blot. {RE}

产品信息

PA5-18598

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.001-0.1 µg/mL

免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

Assay-dependent

免疫组织化学（多聚甲醛固定） (IHC (PFA))

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免疫细胞化学 (ICC/IF)

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse

宿主/亚型

Goat / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide sequence (DGEVIKESKQEHKD) corresponding to the C-terminus amino acids of GFAP

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Ammonium sulfate precipitation

保存液

TBS, pH 7.3, with 0.5% BSA

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

RRID

AB_10984384

产品详细信息

This antibody is predicted to react with canine and rat based on sequence homology.

This antibody is tested in Peptide ELISA: antibody detection limit dilution 128,000.

靶标信息

GFAP (Glial fibrillary acidic protein) is a member of the class III intermediate filament protein family. GFAP is heavily and specifically expressed in astrocytes and certain astroglia of the central nervous system, in satellite cells of peripheral ganglia, and in non-myelinating Schwann cells of peripheral nerves. In addition, neural stem cells strongly express GFAP. Antibodies to GFAP are very useful as markers of astrocytic cells. In addition, many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes. GFAP is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing of the GFAP gene results in multiple transcript variants encoding distinct isoforms.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

A Three-Dimensional Brain-on-a-Chip Using Human iPSC-Derived GABAergic Neurons and Astrocytes.

Methods in molecular biology (Clifton, N.J.)

Liu L,Koo Y,Russell T,Yun Y

PA5-18598 was used in Immunocytochemistry to describe a method to construct a brain-on-a-chip with human induced pluripotent stem cell (iPSC)-derived neurons and astrocytes to simulate human brain behavior.

Fri Jun 24 00:00:00 EDT 2022

Beneficial contribution of induced pluripotent stem cell-progeny to Connexin 47 dynamics during demyelination-remyelination.

Glia

Mozafari S,Deboux C,Laterza C,Ehrlich M,Kuhlmann T,Martino G,Baron-Van Evercooren A

PA5-18598 was used in Immunohistochemistry to investigate the expression dynamics of the oligodendrocyte specific connexin 47 (Cx47) and to some extent that of astrocyte Cx43, and whether this dynamic could be modulated by grafted induced pluripotent stem cell (iPSC)-neural progeny.

Sat May 01 00:00:00 EDT 2021

Metabolic Profiling of Suprachiasmatic Nucleus Reveals Multifaceted Effects in an Alzheimer's Disease Mouse Model.

Journal of Alzheimer's disease : JAD

Eeza MNH,Singer R,Höfling C,Matysik J,de Groot HJM,Roβner S,Alia A

Published figure using GFAP polyclonal antibody (Product # PA5-18598) in Immunohistochemistry

Mon Sep 06 00:00:00 EDT 2021

Zika Virus Targets Glioblastoma Stem Cells through a SOX2-Integrin α_vβ₅ Axis.

Cell stem cell

Zhu Z,Mesci P,Bernatchez JA,Gimple RC,Wang X,Schafer ST,Wettersten HI,Beck S,Clark AE,Wu Q,Prager BC,Kim LJY,Dhanwani R,Sharma S,Garancher A,Weis SM,Mack SC,Negraes PD,Trujillo CA,Penalva LO,Feng J,Lan Z,Zhang R,Wessel AW,Dhawan S,Diamond MS,Chen CC,Wechsler-Reya RJ,Gage FH,Hu H,Siqueira-Neto JL,Muotri AR,Cheresh DA,Rich JN

PA5-18598 was used in Immunohistochemistry (PFA fixed) to demonstrate that ZIKV preferentially infected and killed GSCs and stem-like cells in medulloblastoma and ependymoma in a SOX2-dependent manner.

Thu Feb 06 00:00:00 EST 2020

Three-dimensional brain-on-chip model using human iPSC-derived GABAergic neurons and astrocytes: Butyrylcholinesterase post-treatment for acute malathion exposure.

PloS one

Liu L,Koo Y,Russell T,Gay E,Li Y,Yun Y

PA5-18598 was used in Immunocytochemistry to develop a three-dimensional (3D) brain-on-chip platform with human induced pluripotent stem cell (iPSC)-derived neurons and astrocytes to simulate human brain behavior.

Fri Jul 10 00:00:00 EDT 2020

Three-dimensional brain-on-chip model using human iPSC-derived GABAergic neurons and astrocytes: Butyrylcholinesterase post-treatment for acute malathion exposure.

PloS one

Liu L,Koo Y,Russell T,Gay E,Li Y,Yun Y

Fri Jul 10 00:00:00 EDT 2020

生物信息学

蛋白别名: Astrocyte or Intermediate Filament Protein; FLJ45472; GFAP; GFAP epsilon; Glial fibrillary acidic protein; Glial Fibrillary Acidic Protein (GFAP); glial fibrillary acidic protein alpha; intermediate filament; intermediate filament protein

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基因别名: AI836096; ALXDRD; GFAP

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UniProt ID: (Human) E9PAX3, (Rat) P47819, (Mouse) P03995

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Entrez Gene ID: (Human) 2670, (Rat) 24387, (Mouse) 14580

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功能

glycoprotein binding integrin binding structural constituent of cytoskeleton protein binding kinase binding structural molecule activity

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